StraightFrom® LRSC MicroBeads

Cell isolation directly from LRSC 

  • Highly pure cells straight from LRSC
  • No density gradient centrifugation, no erythrocyte lysis
  • Easy and fast protocol with only a few handling steps

StraightFrom MicroBeads allow magnetic cell separation directly from Leukoreduction system chambers (LRSCs). Leukocyte subsets can be purified using either the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus or manual separators. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.

Streamlining cell separation from LRSCs

LRSCs arise after routine donor plateletpheresis procedures, during which unwanted leukocytes are filtered out into the LRSC, also known as buffy cone. As LRSCs contain highly concentrated leukocytes in a relatively small volume, they are an ideal source for leukocytes. StraightFrom LRSC MicroBead Kits enable the separation of various leukocyte subsets directly from LRSCs via manual or semi-automated cell separation with the MultiMACS Cell24 Separator Plus.

Automated cell isolation with the MultiMACS Cell24 Separator Plus

Automated cell isolation with the MultiMACS™ Cell24 Separator Plus
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The MultiMACS Cell24 Separator Plus is ideally suited to process an entire LRSC in a single run with the StraightFrom LRSC MicroBead Kits. The 30-minute isolation procedure results in cell populations with high purities and recovery rates. With 12 LS Columns (included in the kit), cell isolation directly from LRSC becomes as easy as it gets. The instrument allows for time saving when processing large sample volumes, while providing reliability and reproducibility in day-to-day cell separation. 

Manual cell isolation

Manual cell isolation together with the LS columns
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StraightFrom LRSC MicroBead Kits are suited for the separation of cell populations directly from an entire or partial LRSC. Together with the LS columns (included in the kit) and magnetic separators such as the QuadroMACS™ Separator, the manual method allows flexible handling of samples of different sizes and donors.

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