Annexin V MicroBead Kit

Annexin V MicroBead Kit

The Annexin V MicroBead Kit was developed for positive selection or depletion of cells based on their ability to bind Annexin V. The Annexin V MicroBead Kit includes Annexin V MicroBeads and Annexin V Binding Buffer.

Background information

In most normal, viable eukaryotic cells the negatively charged phospholipid phosphatidylserine (PS) is located in the cytosolic leaflet of the plasma membrane lipid bilayer
1
. PS redistribution from the inner to the outer leaflet is an early and widespread event during apoptosis
1,2
. However, in necrosis, PS becomes accessible due to the disruption of membrane integrity
2
. Apart from necrosis and apoptosis, PS also becomes accessible in activated platelets
3
, in certain cell anomalies such as sickle cell anaemia
4
, in erythrocyte senescence
5
, upon degranulation of mast cells
10
, and in certain stages of B cell differentiation
11
. PS exposure also serves as a trigger for the recognition and removal of cells by macrophages
6,9
.
Annexin V is a 35 kDa phospholipid-binding protein and a major cell membrane component of macrophages and other phagocytic cell types. Annexin V has a high affinity to PS in the presence of physiological concentrations of calcium (CA
2+
)
7
and has already been used to isolate cells with exposed PS using MACS
®
MicroBeads.
4,8

Applications

The Annexin V MicroBead Kit is used for the isolation of cells with exposed PS, including sickle cells and apoptotic cells. The Annexin V MicroBead Kit has been further applied to the purification of apoptotic and non-apoptotic neutrophils to study the intracellular signaling pathways that regulate neutrophil survival. In a study on the roles of different cytosolic phospholipase A2s in apoptosis, the Annexin V MicroBead Kit has been used for sensitive assessment of PS externalization.

Columns

For positive selection: MS, LS or XS Columns. For depletion: LD, CS or D Columns.
Product options: 1

Product information

Size
Order no.
Price

Annexin V MicroBead Kit

  • For research use only
for 1×
10
9
total cells
130-090-201
-

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