Tumor Cell Isolation Kit, human

Tumor Cell Isolation Kit, human

The Tumor Cell Isolation Kit, human enables the enrichment of human tumor cells from primary specimens using MACS
®
Cell Separation technology.
While depleting tumor associated cells such as lymphocyte subpopulations, fibroblasts, and endothelial cells in a fast and easy protocol, the tumor cells remain untouched, improving any downstream analysis.

Background information

The Tumor Cell Isolation Kit, human has been designed for the enrichment of untouched human tumor cells from primary specimens. During the growth phase in vivo, tumor tissue is vascularized and infiltrated by cells of non-tumor origin, including heterogeneous lymphocyte subpopulations, fibroblasts, and endothelial cells.
The level of infiltration is highly dependent on multiple factors like tumor subtype, growth rate, and affected organ site. However, even when these factors are constant, the amount and composition of infiltrating cells are highly variable, which makes accurate molecular downstream analyses difficult.
The contaminating non-tumor cells lead to hybridization of non-tumor cell derived mRNA molecules to probes on microarrays and a significant reduction of sensitivity caused by measurement of irrelevant signals during next-generation sequencing or proteome analysis. In addition, the culture of human tumor cells is frequently hampered by fibroblasts overgrowing the target cells. For optimal results, the Tumor Cell Isolation Kit, human should be used in combination with the Tumor Dissociation Kit, human (# 130-095-929) and gentleMACS™ Dissociators.

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