Mouse Cell Depletion Kit

Mouse Cell Depletion Kit

The Mouse Cell Depletion Kit has been designed for the enrichment of untouched human cells upon xenotransplantation.

Background information

The Mouse Cell Depletion Kit has been designed for the enrichment of untouched human cells upon xenotransplantation. Human tumor xenografts represent the benchmark for research areas like drug discovery, cancer stem cell biology, and metastasis prediction
1
. During the growth phase
in vivo
, xenografted tissue is vascularized and infiltrated by cells of mouse origin, including heterogeneous lymphocyte subpopulations, fibroblasts, and endothelial cells. The level of infiltration is highly dependent on multiple factors like tumor subtype, growth rate, and region of transplantation. However, even when these factors are kept constant, the amount and composition of infiltrating mouse cells are highly variable, which makes accurate molecular downstream analyses difficult. The contaminating mouse cells lead to cross-hybridization of mouse-derived molecules to human probes on microarrays and a significant reduction of sensitivity caused by measurement of mouse signals during next-generation sequencing or proteome analysis
2
. In addition, the culture of human tumor cells is frequently hampered by murine fibroblasts overgrowing the target cells. For optimal results, the Mouse Cell Depletion Kit should be used in combination with the Tumor Dissociation Kit, human (# 130-095-929).

Columns

LS or autoMACS
®
Columns.
Video

How to deplete mouse cells from human tumor xenografts

Product options: 1

Product information

Size
Order no.
Price

Mouse Cell Depletion Kit

  • For research use only
for
up to
10
9
total cells
130-104-694
-

Related products

8 products available | view all