Carcinoma Cell Enrichment Kit, human

Carcinoma Cell Enrichment Kit, human

The Carcinoma Cell Enrichment Kit was developed for more than a 10,000-fold the enrichment of disseminated epithelial tumor cells from peripheral blood, bone marrow, or lymphoid tissue by the positive selection of cytokeratin 7/8 expressing cells. For convenient subsequent immunocytochemical detection the Carcinoma Cell Enrichment and Detection Kit can be used.

Background information

Most malignant cells which have their origin in epithelial tissue express cytokeratin and can be recognized by an anti-cytokeratin antibody
1
. With the Carcinoma Cell Enrichment Kit, disseminated epithelial tumor cells can rapidly and efficiently be enriched using MACS Cytokeratin MicroBeads, e.g. from a frequency of 1 tumor cell in 10
7
leukocytes to a frequency of 1 tumor cell per 10
3
leukocytes. Thus, large patient samples can be narrowed and screened for disseminated tumor cells on only a few microscopy slides. Detection rates of occult tumor cells can be increased to more than 80%
1,2
.

Detailed separation procedure

Tumor cells of epithelial origin such as metastatic carcinomas express cytokeratins and can be positively selected by using Anti-Cytokeratin MicroBeads. Cells are permeabilized, fixed, and incubated with Anti-Cytokeratin MicroBeads for direct immunomagnetic labeling of intracellular cytokeratin. The magnetically labeled cells are then enriched using MS or LS Columns.

Downstream applications

Since the separation procedure preserves cell morphology, carcinoma cells can quickly and easily be identified and enumerated by immunocytochemistry, immunofluorescence microscopy, or flow cytometry. Enriched carcinoma cells can also be further analyzed by molecular biology methods. For example, single tumor cells and tumor cell clusters have been enriched for further characterization, e.g. by DNA staining and immunocytochemistry
3,4
.

Columns

MS Columns.
  • Selected References

    1. Heatley, M. et al. (1995) Cytokeratin intermediate filament expression in benign and malignant breast disease. J. Clin. Pathol. 48: 26-32
    2. Weihrauch, M. R. et al. (2002) Immunomagnetic enrichment and detection of micrometastases in colorectal cancer: correlation with established clinical parameters. J. Clin. Oncol. 20: 4338-4343
    3. Weihrauch et al. (2002) Immunomagnetic enrichment and detection of isolated tumor cells in bone marrow of patients with epithelial malignancies. Clin. Exp. Metastasis 19: 617-621
    4. Schmidt, H. et al. (2004) Frequent detection and immunophenotyping of prostate-derived cell clusters in the peripheral blood of prostate cancer patients. Int. J. Biol. Markers 19: 93-99
    5. Schmidt et al. (2006) Asynchronous growth of prostate cancer is reflected by circulating tumor cells delivered from distinct, even small foci, harboring loss of heterozygosity of the PTEN gene. Cancer Res. 66(18): 8959-8965
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Product information

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Order no.
Price

Carcinoma Cell Enrichment Kit, human

  • For research use only
for 1×
10
9
total cells
–3×
10
9
total cells
130-060-101
-