Naive CD8+ T Cell Isolation Kit, human

Naive CD8
+
T Cell Isolation Kit
, human

The Naive CD8
+
T Cell Isolation Kit was developed for the isolation of naive CD8
+
T cells from human PBMCs in a two-step procedure.

Detailed separation procedure

The isolation of naive CD8
+
T cells is performed in a two-step procedure. First, naive T cells are enriched by depletion of non-naive T cells and NK cells, which are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. In a second step, the enriched naive T cells are labeled with CD8 MicroBeads for subsequent positive selection of the CD8
+
naive T cells.

Downstream applications

Naive CD8
+
T cells isolated with the Naive CD8
+
T Cell Isolation Kit can be used for functional T cell studies, e.g., studies on signal transduction or cytokine expression after activation of the naive CD8
+
T cells.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
Product options: 1

Product information

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Order no.
Price

Naive CD8
+
T Cell Isolation Kit
, human

  • For research use only
for 1×
10
9
total cells
130-093-244
-