Naive CD4+ T Cell Isolation Kit, mouse

Naive CD4
+
T Cell Isolation Kit
, mouse

The Naive CD4
+
T Cell Isolation Kit, mouse has been developed for the isolation of untouched naive CD4
+
T helper cells from suspensions of mouse spleen cells or lymph node cells.

Detailed separation procedure

Mouse naive CD4
+
T cells are isolated by depletion of memory CD4
+
T cells and non-CD4
+
T cells. Single-cell suspensions of mouse spleen or lymph nodes are indirectly magnetically labeled with a cocktail of biotinylated monoclonal antibodies, as primary labeling reagent, and Anti-Biotin MicroBeads, as secondary labeling reagent. Simultaneously, CD44 MicroBeads are added to label memory T cells. No washing steps are required during magnetic labeling. The magnetically labeled non-target cells are depleted by retaining them on a MACS
®
Column in the magnetic field of a MACS Separator, while the unlabeled target cells pass through the column.

Applications

Naïve CD4
+
T cells are perfect for use in
i n vitro
analysis of T cell differentiation, adoptive transfer experiments, studies on signal requirements for CD4
+
T cell activation and proliferation, and for studies on regulation of CD4
+
T cell cytokine expression.

Columns

LS or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

Naive CD4
+
T Cell Isolation Kit
, mouse

  • For research use only
for 1×
10
9
cells
130-104-453
-

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