CD8a (Ly-2) MicroBeads, mouse

CD8a (Ly-2) MicroBeads, mouse

Mouse CD8a (Ly-2) MicroBeads have been developed for positive selection or depletion of mouse CD8a
+
 T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

Background information

The CD8a antigen is expressed on most thymocytes, almost all cytotoxic T cells, and on subpopulations of dendritic cells. CD8a functions as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on cytotoxic CD8a
+
 T cells.

Downstream applications

CD8a (Ly-2) MicroBeads, mouse were developed for the isolation of mouse CD8
+
T cells from various lymphoid and non-lymphoid tissues or from peripheral blood for the subsequent functional and phenotypical analysis of T cells 
in vitro
 and
in vivo
Examples for downstream applications include:
  • In vitro and in vivo studies on protective immune responses against parasites or allergens
  • Adoptive transfer into immunodeficient and virus-infected mice
  • Isolation of highly pure CD8+ T cells from CNS of MHV-infected mice for evaluation of their chemokine expression patterns
  • In vitro activation and expansion of CD8+ T cells (e.g. with the T Cell Activation/Expansion Kit, mouse)
  • Antigen-specific stimulation of CD8+ T cells (e.g. with PepTivator® Peptide Pools)
  • Analysis of cytokine secretion and surface marker expression (e.g. with REAfinity™ Recombinant Antibodies)
  • Examination of the regulatory role of cytokines in autoimmune disease and infection

Columns

For positive selection: MS, LS, or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD8a (Ly-2) MicroBeads, mouse

  • For research use only
for 2×
10
9
total cells
130-117-044
-

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