CD8 MicroBeads, human

CD8 MicroBeads, human

CD8 MicroBeads were developed for the positive selection or depletion of human cytotoxic CD8
+
T cells from different cell sources. The most commonly used cell sources are peripheral blood and cord blood. In addition, cytotoxic T cells have also been isolated from lymph nodes, thymus, skin biopsies
1
, and spleen.

Background information

The CD8 antigen forms a complex together with the T cell receptor and acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells. The CD8 molecule consists of either an α/β heterodimer or an α/α homodimer. It is expressed strongly on cytotoxic T cells and dimly on a subset of NK cells. CD8 is found on most thymocytes and on about one third of all peripheral blood T cells. CD8
+
cytotoxic T cells play an important role in the killing of virus-infected cells and tumor cells.

Downstream applications

Isolation or depletion of CD8
+
cytotoxic T cells is performed in many different research fields such as infectious diseases, autoimmune diseases, allergy, and asthma, as well as tumor immunology.
Cytotoxic T cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays
2,9
and cytotoxicity assays
2,3
, but also for the analysis of
in vitro
cytokine production
4
. CD8 MicroBeads have also been used for the depletion of CD8
+
T cells from human PBMCs for immune reconstitution experiments in SCID mice.
5
In combination with CD4 MultiSort MicroBeads, CD8 MicroBeads have been used for the isolation of CD4
+
CD8
+
double-positive thymocytes.
8
Other examples include various studies on viral infections, e.g.,
in vitro
infections of CD8
+
cells with HIV
6
, or monitoring of immune abnormalities in children of HIV-infected mothers
7
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, CS, D, or autoMACS Columns.
Product options: 2

Product information

Size
Order no.
Price

CD8 MicroBeads, human

  • For research use only
for 1×
10
9
total cells
130-045-201
-

CD8 MicroBeads, human - lyophilized

  • For research use only
for 1×
10
9
total cells
130-097-057
-

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