CD8+ CD45RA+ Effector T Cell Isolation Kit, human

CD8
+
CD45RA
+
Effector T Cell Isolation Kit
, human

The CD8
+
CD45RA
+
Effector T Cell Isolation Kit has been developed for the positive selection of CD8
+
CD45RA
+
effector T cells from human PBMCs.

Background information

The CD8
+
CD45RA
+
Effector T Cell Isolation Kit is ideal for the isolation of cytotoxic T cells that express high levels of perforin and granzyme B
1
. Isolated cells can be analyzed for interactions with other cell types and degranulation or secretion of effector molecules.

Detailed separation procedure

The isolation of CD8
+
CD45RA
+
effector T cells is performed in a two-step procedure. First, non-target cells are indirectly magnetically labeled for depletion with biotin-conjugated antibodies against CD45RO and CD56 and an APC-conjugated antibody against CD197 (CCR7), as well as Anti-APC MicroBeads and Anti-Biotin MicroBeads. In the second step, the CD8
+
CD45RA
+
effector T cells are directly labeled with CD8 MicroBeads and isolated by positive selection from the pre-enriched cell fraction.

Columns

For positive selection: MS, LS, or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD8
+
CD45RA
+
Effector T Cell Isolation Kit
, human

  • For research use only
for 2×
10
9
total cells
130-094-485
-

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