CD4+ T Cell Isolation Kit, mouse

CD4
+
T Cell Isolation Kit
, mouse

The CD4
+
T Cell Isolation Kit, mouse was developed for the untouched isolation of CD4
+
T cells from single-cell suspensions of mouse spleen and/or lymph nodes.
This kit uses the new fast mouse protocol and replaces the CD4
+
T Cell Isolation Kit II (# 130-095-248), allowing isolation of CD4
+
T cells in just 18 minutes.

Detailed separation procedure

CD4
+
T cells are isolated by depletion of non CD4
+
T cells using a cocktail of biotin-conjugated antibodies against CD8a, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, Ter-119 and TCRγ/δ as primary labeling reagent. The cells are then magnetically labeled with Anti-Biotin MicroBeads. The magnetically labeled non-target cells are depleted by retaining them on a MACS
®
Column in the magnetic field of a MACS Separator, while the unlabeled target cells pass through the column.

Downstream applications

The CD4
+
T Cell Isolation Kit, mouse is used where untouched CD4
+
T cells are desired or where further magnetic separation into T cell subsets is required.

Columns

LS or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

CD4
+
T Cell Isolation Kit
, mouse

  • For research use only
for 1×
10
9
cells
130-104-454
-

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