CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit II, human

CD4
+
CD25
+
CD127
dim/-
Regulatory T Cell Isolation Kit II
, human

The CD4
+
CD25
+
CD127
dim/–
Regulatory T Cell Isolation Kit II was developed for the isolation of CD4
+
CD25
+
CD127
dim/–
Treg cells from human PBMCs. The isolation is performed in a two-step procedure.

Background information

CD127, the α-chain of the IL-7 receptor, is expressed on the majority of mature T cells and plays an important role in their proliferation and differentiation. However, on regulatory T cells CD127 is absent and its expression inversely correlates with FoxP3 expression. Thus, CD127 can be used as an additional marker to discriminate between human regulatory and activated T cells.

Detailed separation procedure

First, the isolation of CD4
+
CD25
+
CD127
dim/–
regulatory T cells was performed with a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for the depletion of non-CD4
+
and CD127
high
cells. In the second step, the flow-through fraction of pre-enriched CD4
+
CD127
dim/–
T cells is labeled with CD25 MicroBeads for subsequent positive selection of CD4
+
CD25
+
CD127
dim/–
regulatory T cells.

Downstream applications

Isolation of CD4
+
CD25
+
CD127
dim/–
regulatory T cells from human PBMCs for further phenotypical or functional characterization.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

CD4
+
CD25
+
CD127
dim/-
Regulatory T Cell Isolation Kit II
, human

  • For research use only
for 1×
10
9
total cells
130-094-775
-

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