CD294 (CRTH2) MicroBead Kit, human

CD294 (CRTH2) MicroBead Kit, human

The CD294 (CRTH2) MicroBead Kit was developed for the positive selection or depletion of CD294
+
cells from human PBMCs.

Background information

CD294, also known as CRTH2, is a receptor for prostaglandin D
2
. CD294 is expressed on T helper type 2 (T
H
2) cells, a subpopulation of CD4
+
T cells, but is not present on T
H
1 cells. CD294 is highly expressed on peripheral blood basophils and eosinophils. Furthermore, it is present on a small population of CD8
+
T cells as well as on a subpopulation of dendritic cells.
For isolation of the CD294
+
T helper cell subset, the CD294 (CRTH2) MicroBead Kit can be combined with the CD4
+
T Cell Isolation Kit or the CD4 MultiSort Kit. A special protocol is available.

Detailed separation procedure

The CD294
+
cells are fluorescently and indirectly magnetically labeled with CD294-PE and Anti-PE MicroBeads.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, CS, D, or autoMACS Columns.
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