CD11b (Microglia) MicroBeads, human and mouse

CD11b (Microglia) MicroBeads, human and mouse

CD11b (Microglia) MicroBeads have been developed for the positive isolation of primary mouse and human CD11b
+
microglia. The purification protocol takes approx. one hour.

Background information

Microglia, often called brain macrophages, are the resident immune effector cells in the central nervous system (CNS). In addition, activated microglia serve as the major antigen-presenting cells in the CNS. They are morphologically, immunophenotypically, and functionally related to cells of the monocyte/macrophage lineage.
This detailed protocol using CD11b (Microglia) MicroBeads allows the fast isolation of primary microglia within 1 hour. The use of the MACS Neural Tissue Dissociation Kit (P) prior to the purification leads to highest yield and viability.
For the isolation of microglia from myelin-containing tissue samples (human tissue or adult mouse or rat), the use of Myelin Removal Beads II is highly recommended.

Downstream applications

CD11b (Microglia) MicroBeads have been used to isolate CD11b
+
cells from single-cell suspensions of brain tissue
3-6
.

Columns

MS, LS, XS, or autoMACS
®
Columns.
Video

How to isolate pure viable microglia

Watch this hands-on protocol showing you how to isolate microglial cells from adult mouse brain. The Miltenyi Biotec protocol is faster, standardized, reproducible, and easy to scale up.

Product options: 2

Product information

Size
Order no.
Price

CD11b (Microglia) MicroBeads, human and mouse - small size

  • For research use only
for 1×
10
8
total cells
130-093-636
-

CD11b (Microglia) MicroBeads, human and mouse

  • For research use only
for 1×
10
9
total cells
130-093-634
-

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