Anti-AN2 MicroBeads, human and mouse

Anti-AN2 MicroBeads, human and mouse

Anti-AN2 MicroBeads have been developed for positive selection or depletion of murine or human AN2
+
glial cells.

Background information

AN2
1
is the mouse homolog of the rat NG2 and human MCSP protein. NG2 is a chondroitin sulfate glycoprotein that is expressed by glial progenitor cells in the developing and adult central nervous system (CNS) and by immature Schwann cells.
NG2/AN2
+
glia has been identified as a fourth major glial cell type in the mammalian CNS that is distinct from other cell types
2
. NG2/AN2
+
cells are precursors of oligodendrocytes and some protoplasmic astrocytes in the grey matter
3
. NG2/AN2 expression is down-regulated before the cells undergo terminal differentiation. However, a population of NG2/AN2
+
cells remain in the white and grey matter after oligodendrocytes are generated
4-6
.
NG2/AN2
+
glial cells participate in neuron-glial circuits by receiving presynaptic input from neurons and responding to neurotransmitters released by synapses
7
.
Many highly migratory neural tumors including melanomas express NG2/AN2.

Columns

For positive selection: MS, LS, or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.
  • Selected References

    1. Stegmuller, J. et al. (2002) AN2, the mouse homologue of NG2, is a surface antigen on glial precursor cells implicated in control of cell migration. J. Neurocytol. 31: 497-505
    2. Trotter, J. et al. (2010) NG2 cells: Properties, progeny and origin. Brain Res. Rev. 63: 72-82
    3. Zhu, X. et al. (2008) NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development 132: 145-157
    4. Diers-Fenger, M. et al. (2001) AN2/NG2 protein-expressing glial progenitor cells in the murine CNS: isolation, differentiation, and association with radial glia. Glia 34: 213-228
    5. Peters, A. (2004) A fourth type of neuroglial cell in the adult central nervous system. J. Neurocytol. 33: 345-357
    6. Zhu, X. et al. (2011) Age-dependent fate and lineage restriction of single NG2 cells. Development 138: 745-753
    7. Wigley, R. et al. (2007) Morphological and physiological interactions of NG2-glia with astrocytes and neurons. J. Anat. 210: 661-670
    8. Nishiyama, A. et al. (2009) Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity. Nat. Rev. Neurosci. 10: 9-22
  • Brochures and posters

Product options: 2

Product information

Size
Order no.
Price

Anti-AN2 MicroBeads, human and mouse - small size

  • For research use only
for 1×
10
8
total cells
130-097-171
-

Anti-AN2 MicroBeads, human and mouse

  • For research use only
for 1×
10
9
total cells
130-097-170
-