Pan Monocyte Isolation Kit, human

Pan Monocyte Isolation Kit, human

The Pan Monocyte Isolation Kit is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. This Kit allows the simultaneous enrichment of classical (CD14
++
CD16
), non classical (CD14
+
CD16
++
) and intermediate (CD14
++
CD16
+
) monocytes.
1
Highly pure unlabeled monocytes are obtained by depletion of the magnetically labeled cells.

Detailed separation procedure

Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads.

Downstream applications

  • Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
  • Studies on monocyte activation, differentiation, cytokine secretion etc.
  • Studies on signal transduction in monocytes.
  • Studies on antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
  • In vitro differentiation of blood monocytes into dendritic cells.

Columns

MS, LS, XS, or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

Pan Monocyte Isolation Kit, human

  • For research use only
for 1×
10
9
total cells
130-096-537
-

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