Classical Monocyte Isolation Kit, human

Classical Monocyte Isolation Kit, human

The Classical Monocyte Isolation Kit provides fast and consistent isolation of untouched classical ( CD14
++
CD16
) monocytes from peripheral blood mononuclear cells (PBMCs) for further functional studies. Depletion of non-monocytes is optimal and provides a highly pure population of label-free classical monocytes. This product includes an optional thrombocytes removal reagent to further reduce platelet contamination.

Background information

Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled monocytes is achieved by depletion of the magnetically labeled cells.

Downstream applications

  • Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
  • Studies on monocyte activation, differentiation, cytokine secretion etc.
  • Studies on compound or antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
  • In vitro
    • differentiation of blood monocytes into dendritic cells or macrophages.

Columns

MS Columns, LS Columns, autoMACS
®
Columns, or Multi-24 Column Blocks.
Product options: 1

Product information

Size
Order no.
Price

Classical Monocyte Isolation Kit, human

  • For research use only
for 1×
10
9
total cells
130-117-337
-

Related products

10 products available | view all