CD115 MicroBead Kit, mouse

CD115 MicroBead Kit, mouse

The CD115 MicroBead Kit has been developed for positive selection of CD115
+
mouse cells from lymphoid tissues.

Background information

CD115 is expressed on monocytes, macrophages, osteoclasts, as well as on common dendritic cell precursors and macrophage/dendritic cell precursors. CD115 has been proposed as a marker for the standardized identification and analysis of mouse monocytes.
1

Detailed separation procedure

In order to avoid non-specific binding of the antibody, the single-cell suspension is pre-incubated with FcR Blocking Reagent. Target cells are then labeled with a biotinylated CD115 antibody. Subsequently, CD115
+
cells are magnetically labeled with Anti-Biotin MicroBeads. After the separation over a MACS Column, the CD115
+
cells are eluted as the positive fraction.

Downstream applications

Isolated mouse CD115
+
cells , e.g. from bone marrow, can be used for
in vitro
analysis, differentiation studies, co-culture, or adoptive transfer experiments.

Columns

MS, LS, or autoMACS
®
Columns.
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