Indirect CD133 MicroBead Kit, human

Indirect CD133 MicroBead Kit, human

The Indirect CD133 MicroBead Kit was developed for positive selection or depletion of CD133
+
cells, using a two-step procedure involving indirect magnetic labeling.
  • Hematopoietic stem cells can be isolated from peripheral blood, cord blood, bone marrow, or leukapheresis product.
  • Neural progenitor cells can be isolated from single-cell suspensions from primary neural tissues or cell lines.
  • ES and iPS cell–derived neural, endothelial, or hematopoietic progenitors can be isolated from differentiated ES or iPS cell cultures1 .
  • Cancer stem cells can be isolated from single-cell suspensions from primary tumor tissue or cell lines.

Background information

CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen.
2,3
It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34
cells
4
as well as on a subset of CD34
bright
stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood
5
. CD133 has also been found to be expressed on circulating endothelial progenitor cells;
6,7
fetal neural stem cells;
8,9
other tissue-specific stem cells, such as renal
10
, prostate
11
, and corneal
12
stem cells; cancer stem cells from tumor tissues; as well as ES and iPS cell-derived cells.
There is a growing interest in CD133 antigen expressing stem cells from normal blood or bone marrow in the field of regenerative medicine, for example bone marrow-derived CD133
+
stem cells in cardiovascular
13-17
, liver, or peripheral artery diseases
18-20
.
The CD133 antibody included in the kit recognizes epitope CD133/1. For quality control staining of CD133-separated cells, the use of CD133/2 (293C3)-PE or -APC is recommended.

Downstream applications

Isolated from hematopoietic sources, CD133
+
cells can become adherent and are reported to become CD133-negative during culture.
21,22
These adherent cells can then in turn give rise to nonadherent CD133
+
cells that are able to differentiate to both hematopoietic and nonhematopoietic cell types.
23
CD133
+
cells have shown a capacity for tissue differentiation, including to neural lineages
24
. CD133
+
isolated from fetal liver
25
, umbilical cord blood
26
, bone marrow
27
, mobilized blood
28
, and skin
29
are capable of in vitro differentiation to neuronal cells as well as to astrocytes,
25,26,28
oligodendrocytes,
26,28
and glial cells.
26
CD133
+
cells isolated from human fetal brain
8,9,30-32
were able to form self-renewing neurospheres in vitro, and to differentiate into neurons
8,32
and glia
19,23
. When injected into mice, human CD133
+
cells differentiated into fully integrated neurones and glial cells
9,30
as well as astrocytes and endothelial cells
29
. The CD34
+
CD133
+
cell population, which includes CD34
+
CD38
cells, was shown to be capable of repopulating NOD/SCID mice
33
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, CS, D, or autoMACS Columns.
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