Indirect CD133 MicroBead Kit, human

Indirect CD133 MicroBead Kit, human

The Indirect CD133 MicroBead Kit was developed for positive selection or depletion of CD133
cells, using a two-step procedure involving indirect magnetic labeling.
  • Hematopoietic stem cells can be isolated from peripheral blood, cord blood, bone marrow, or leukapheresis product.
  • Neural progenitor cells can be isolated from single-cell suspensions from primary neural tissues or cell lines.
  • ES and iPS cell–derived neural, endothelial, or hematopoietic progenitors can be isolated from differentiated ES or iPS cell cultures1 .
  • Cancer stem cells can be isolated from single-cell suspensions from primary tumor tissue or cell lines.

Background information

CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen.
It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34
as well as on a subset of CD34
stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood
. CD133 has also been found to be expressed on circulating endothelial progenitor cells;
fetal neural stem cells;
other tissue-specific stem cells, such as renal
, prostate
, and corneal
stem cells; cancer stem cells from tumor tissues; as well as ES and iPS cell-derived cells.
There is a growing interest in CD133 antigen expressing stem cells from normal blood or bone marrow in the field of regenerative medicine, for example bone marrow-derived CD133
stem cells in cardiovascular
, liver, or peripheral artery diseases
The CD133 antibody included in the kit recognizes epitope CD133/1. For quality control staining of CD133-separated cells, the use of CD133/2 (293C3)-PE or -APC is recommended.

Downstream applications

Isolated from hematopoietic sources, CD133
cells can become adherent and are reported to become CD133-negative during culture.
These adherent cells can then in turn give rise to nonadherent CD133
cells that are able to differentiate to both hematopoietic and nonhematopoietic cell types.
cells have shown a capacity for tissue differentiation, including to neural lineages
. CD133
isolated from fetal liver
, umbilical cord blood
, bone marrow
, mobilized blood
, and skin
are capable of in vitro differentiation to neuronal cells as well as to astrocytes,
and glial cells.
cells isolated from human fetal brain
were able to form self-renewing neurospheres in vitro, and to differentiate into neurons
and glia
. When injected into mice, human CD133
cells differentiated into fully integrated neurones and glial cells
as well as astrocytes and endothelial cells
. The CD34
cell population, which includes CD34
cells, was shown to be capable of repopulating NOD/SCID mice


For positive selection: MS, LS, XS, or autoMACS
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected References

    1. Galic, Z. et al. (2006) T lineage differentiation from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 103: 11742-11747
    2. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
    3. Piechaczek, C. (2001) CD133. J. Biol. Regul. Homeost. Agents 15: 101-102
    4. Gallacher, L. et al. (2000)
      Isolation and characterization of human CD34
      and CD34
      hematopoietic stem cells using cell surface markers AC133 and CD7.
      Blood 95(ARVO Annual Meeting Abstract): 2813-2820
    5. Bühring, H. J. et al. (1999) Expression of novel surface antigens on early hematopoietic cells. Ann. N. Y. Acad. Sci. 872: 25-39
    6. Gehling, U. M. et al. (2000)
      In vitro
      differentiation of endothelial cells from AC133-positive progenitor cells.
      Blood 95: 3106-3112
    7. Peichev, M. et al. (2000)
      Expression of VEGFR-2 and AC133 by circulating human CD34
      cells identifies a population of functional endothelial precursors.
      Blood 95: 952-958
    8. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
    9. Cummings, B. J. et al. (2005) Human neural stem cells differentiate and promote locometer recovery in spinal cord-injured mice. Proc. Natl. Acad. Sci. U.S.A. 102: 14069-14074
    10. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
    11. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
    12. Thill, M. et al. (2004)
      Identification of a population of CD133
      precursor cells in the stroma of human cornea.
      Invest. Ophthalmol. Vis. Sci. 45: 3519
    13. Stamm, C. et al. (2003) Autologous bone-marrow stem-cell transplantation for myocardial regeneration. Lancet 4, 361(9351): 45-46
    14. Stamm, C. et al. (2007)
      Intramyocardial delivery of CD133
      bone marrow cells and coronary artery bypass grafting for chronic ischemic heart disease: safety and efficacy studies.
      J. Thorac. Cardiovasc. Surg. 133: 717-725
    15. Klein (2007) Eur. Cardiovasc. Dis. 1: 123-125
    16. Klein, H. M. et al. (2007)
      Intramyocardial implantation of CD133
      stem cells improved cardiac function without bypass surgery.
      Heart Surg. Forum 10: E66-69
    17. Bartunek et al. (2005) Circulation 30: 178-183
    18. am Esch, J. S. 2nd et al. (2005)
      Portal application of autologous CD133
      bone marrow cells to the liver: a novel concept to support hepatic regeneration.
      Stem Cells 23(4): 463-470
    19. Fürst, G. et al. (2007)
      Portal vein embolization and autologous CD133
      bone marrow stem cells for liver regeneration: initial experience.
      J. Immunother. 243(1): 171-179
    20. Cañizo et al. (2007)
      Peripheral endothelial progenitor cells (CD133
      ) for therapeutic vasculogenesis in a patient with critical limb ischemia. One year follow-up.
      Cytotherapy 9(1): 99-102
    21. Kuçi et al. (2003)
      Identification of a novel class of human adherent CD34
      stem cells that give rise to SCID-repopulating cells.
      Blood 101: 869-876
    22. Kuçi et al. (2008)
      in vitro
      generation of adult multipotent cells from mobilized peripheral blood CD133
      Cell Prolif. 41: 12-27
    23. Kuçi et al. (2003) MACS&more 7(1): 6-8
    24. Kuçi et al. (2004) 2nd Intl. Meeting, Stem Cell Network, North-Rhine Westphalia. : abstract
    25. Hao, H. N. et al. (2003)
      Fetal human hematopoietic stem cells can differentiate sequentially into neural stem cells and then astrocytes
      in vitro
      J. Hematother. Stem Cell Res. 12: 23-32
    26. Jang, Y. K. et al. (2004) Retinoic acid-mediated induction of neurons and glial cells from human umbilical cord-derived hematopoietic stem cells. J. Neurosci. Res. 75: 573-584
    27. Padovan et al. (2003) Expression of neuronal markers in differentiated marrow stromal cells and CD133+ stem-like cells. Cell Transplant 12: 839-848
    28. Piechaczek, C. et al. (2002)
      Differentiation of adult CD133
      cells isolated from peripheral blood into cells with a neural phenotype.
      Stem cell research (customer reports published by Miltenyi Biotec) : 2-3
    29. Belicchi, M. et al. (2004) Human skin-derived stem cells migrate throughout forebrain and differentiate into astrocytes after injection into adult mouse brain. J. Neurosci. Res. 77: 475-486
    30. Tamaki, S. et al. (2002) Engraftment of sorted/expanded human central nervous system stem cells from fetal brain. J. Neurol. Res. 69: 976-986
    31. Kelly, S. et al. (2004) Transplanted human fetal neural stem cells survive, migrate, and differentiate in ischemic rat cerebral cortex. Proc. Natl. Acad. Sci. U.S.A. 101: 11839-11844
    32. Yu, S. et al. (2004)
      Isolation and characterization of the CD133
      precursors from the ventricular zone of human fetal brain by magnetic affinity cell sorting.
      Biotechnol. Lett. 26: 1131-1136
    33. de Wynter, E. A. et al. (1998)
      cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
      Stem Cells 16: 387-396
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Product information

Order no.

Indirect CD133 MicroBead Kit, human

  • For research use only
for 2×
total cells