CD34 MultiSort Kit, human

CD34 MultiSort Kit, human

The CD34 MultiSort Kit has been developed for the positive selection of CD34
+
subpopulations by sequential sorting. CD34
+
hematopoietic stem and progenitor cells can be isolated from
  • peripheral blood,
  • bone marrow aspirate,
  • leukapheresis product,
  • cord blood,
  • differentiated ES and iPS cell cultures1 .

Detailed separation procedure

Using the CD34 MultiSort Kit, in the first step CD34
+
cells are positively selected after labeling with the CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34
+
cells can be magnetically labeled and sorted according to a second antigen. The kit contains CD34 MultiSort MicroBeads, MultiSort Release Reagent, and MultiSort Stop Reagent.
Control staining after separation requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

The CD34 MultiSort Kit is used to isolate specific subsets of CD34
+
hematopoietic stem and progenitor cells. CD34-selected cells can be further sorted according to their expression of, e.g., HLA-DR, CD43, CD45RA, CD61, CD71, CD90 (Thy1), or CD133.

Columns

MS, LS, XS, or autoMACS
®
Columns.
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