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Miltenyi Biotec distribution:
hematopoietic progenitor cells were isolated from non-mobilized human PBMCs using the CD133 MicroBead Kit - Hematopoietic Tissue, labeled with CD34-FITC (# 130-081-001) and CD133/2 (293C3)-PE (# 130-090-853) and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Comparison of CD133+
cell isolations from a debris-rich sample using (A) the CD133 MicroBead Kit (# 130-050-801) or (B) the CD133 MicroBead Kit – Hematopoietic Tissue (# 130-100-830), demonstrating reduced debris carry-over with the latter.