CD105 MultiSort Kit (PE), mouse

CD105 MultiSort Kit (PE), mouse

The CD105 MultiSort Kit (PE), mouse has been developed for the isolation of CD105
+
cells or cell subsets, including
  • vascular endothelial cells from mouse tissue or cell cultures
  • long-term repopulating hematopoietic stem cells (LTR-HSCs) from mouse bone marrow when used in combination with the Anti-Sca-1 MicroBead Kit (FITC).

Background information

CD105, also known as Endoglin, is abundantly expressed on angiogenic endothelial cells. In mouse bone marrow, CD105 is also expressed on a subpopulation of Sca-1
+
hematopoietic stem cells that displays long-term repopulating activity in lethally irradiated mice (LTR-HSCs)
1
.

Detailed separation procedure

In the first step, CD105
+
cells are positively selected after labeling with the CD105 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD105 antibody. The selected CD105
+
cells can be magnetically labeled and sorted according to a second antigen. The kits contains CD105 MultiSort MicroBeads, MultiSort Release Reagent, and the MultiSort Stop Reagent.

Downstream applications

Isolated CD105
+
Sca-1
+
cells can be transplanted into lethally irradiated mice to evaluate the long-term engraftment potential of this cell population.

Columns

MS, LS, or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

CD105 MultiSort Kit (PE), mouse

  • For research use only
for 1×
10
9
total cells
130-092-924
-

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