Myeloid-Derived Suppressor Cell Isolation Kit, mouse

Myeloid-Derived Suppressor Cell Isolation Kit, mouse

The Myeloid-Derived Suppressor Cell Isolation Kit has been developed for the isolation of Gr-1
high
Ly-6G
+
and Gr-1
dim
Ly-6G
myeloid cells from lymphoid tissue.

Background information

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells, characterized as CD11b
+
Gr-1
+
, with a remarkable capacity to induce T cell dysfunction. They arise in various pathological conditions (cancer, infection, autoimmune diseases), where the differentiation of immature myeloid progenitor cells into granulocytes, macrophages, or dendritic cells is partially blocked.
Gr-1
dim
Ly-6G
cells can be further subdivided according to the expression of Ly-6C into Gr-1
dim
Ly-6C
high
Ly-6G
and Gr-1
dim
Ly-6C
low
Ly-6G
cells. The proportion of Ly-6C
high
and Ly-6C
low
cells varies among tumor models.

Detailed separation procedure

The Myeloid-Derived Suppressor Cell Isolation Kit has been developed for the isolation of Gr-1
high
Ly-6G
+
and Gr-1
dim
Ly-6G
myeloid cells.
First, the cell suspension is incubated with an Anti-Ly-6G-Biotin antibody and Anti-Biotin MicroBeads. The cells are subsequently applied to a MACS Column, which retains the magnetically labeled Gr-1
high
Ly-6G
+
cells. The unlabeled cells flow through; this cell fraction contains Gr-1
dim
Ly-6G
myeloid cells. The magnetically labeled Gr-1
high
Ly-6G
+
cells can be eluted as the positively selected cell fraction and are further purified by applying them to a second MACS Column.
Second, the pre-enriched Gr-1
dim
Ly-6G
subpopulation is further purified. To this end, the cells are incubated with an Anti-Gr-1-Biotin antibody and Streptavidin MicroBeads, and isolated by positive selection over two MACS Columns.

Downstream applications

MDSCs isolated with this kit can be used for functional analyses, such as T cell activation and effector function in experimental animal models. Isolated MDSCs are also suitable for, e.g., phenotypical analysis or downstream gene expression profiling.

Columns

For the first magnetic separation (depletion): LS or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

Myeloid-Derived Suppressor Cell Isolation Kit, mouse

  • For research use only
for 2×
10
9
total cells
130-094-538
-

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