CD16 MicroBeads, human

CD16 MicroBeads, human

CD16 MicroBeads have been developed for the isolation of eosinophils by depletion of neutrophils from granulocyte-enriched cell fractions from peripheral blood. Alternatively, neutrophils can be isolated by positive selection. Peripheral blood and various tissues (e.g., bone marrow, nasal tissue) are suitable cell sources.

Background information

The CD16 antigen, which is a low-affinity Fc receptor for aggregated IgG, is expressed on neutrophils, NK cells, and macrophages. CD16 MicroBeads have also been used to deplete NK cells.

Downstream applications

Eosinophils isolated with CD16 MicroBeads are used for the functional analysis of surface molecules
1,3
or for studies on signal transduction
7
, chemotaxis
1,6
, cytokine expression
3,5
, degranulation
4
, and apoptosis
7
.

Columns

LD, CS, D, or autoMACS
®
Columns.
  • Selected References

    1. Blom, M. et al. (1995) Eosinophils isolated with two different methods show different characteristics of activation. J. Immunol. Methods 178: 183-193
    2. Hansel, T. T. et al. (1991) An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils. J. Immunol. Methods 145: 105-110
    3. Levi-Schaffer, F. et al. (1995) Association of granulocyte-macrophage colony-stimulating factor with the crystalloid granules of human eosinophils. Blood 85: 2579-2586
    4. Simon, H. U. et al. (1994) Functional platelet-activating factor receptors are expressed by monocytes and granulocytes but not by resting or activated T and B lymphocytes from normal individuals or patients with asthma. J. Immunol. 153: 364-377
    5. Sriramarao, P. et al. (1996) E-selectin preferentially supports neutrophil but not eosinophil rolling under conditions of flow in vitro and in vivo. J. Immunol. 157: 4672-4680
    6. Motegi, Y. and Kita, H. (1998) Interaction with secretory component stimulates effector functions of human eosinophils but not of neutrophils. J. Immunol. 161: 4340-4346
    7. Yousefi, S. et al. (1996) Requirement of Lyn and Syk tyrosine kinases for the prevention of apoptosis by cytokines in human eosinophils. J. Exp. Med. 183: 1407-1414
Product options: 1
130-045-701

CD16 MicroBeads, human

for 2×
10
9
total cells
-