Basophil Isolation Kit II, human

Basophil Isolation Kit II, human

The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs.

Detailed separation procedure

Non-basophils (i.e. T cells, NK cells, B cells, monocytes, dendritic cells, erythroid cells, platelets, neutrophils, and eosinophils) are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure basophils is achieved by depletion of the magnetically labeled cells.

Downstream applications

Isolated basophils have been used for phenotypical characterization
3
, for histamine, leukotriene, and cytokine
1, 4, 5, 6
release assays, for molecular studies
1, 2, 5
, protein analysis
4, 5
, and other applications.

Columns

LS, XS, or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

Basophil Isolation Kit II, human

  • For research use only
for 2×
10
9
total cells
130-092-662
-

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