Neural Crest Stem Cell MicroBeads, human

Neural Crest Stem Cell MicroBeads, human

The Neural Crest Stem Cell MicroBeads were developed for the positive selection or depletion of neural crest stem cells differentiated from pluripotent stem cells. A method for
in vitro
differentiation of neural crest stem cells from human iPS cells is available in the library section.

Background information

CD271, also known as LNGFR (low-affinity nerve growth factor receptor), NGFR (nerve growth factor receptor), or p75NTR (neurotrophin receptor), belongs to the tumor necrosis factor receptor superfamily. CD271 was initially described to be expressed on cells of the nervous system and was suggested to be involved in the development, survival and differentiation of neural cells.
1
CD271 can be found in the central and peripheral nervous system on autonomic and sensory neurons
2
as well as on glial cells, including oligodendrocytes
3
, astrocytes
4
, Schwann cells
5,6
, and neural crest stem cells
7
. Neural crest stem cells can be induced from pluripotent stem cells by synergistic action of two inhibitors of SMAD signaling, Noggin and SB431542
8,9
or alternatively, by a single small molecule dorsomorphin blocking signalling of several (TGF-β) superfamily receptors
10
. CD271
+
neural crest stem cells can be selected ten days post-induction. They show expression of typical neural crest markers (HNK1, AP2) and they can be differentiated to homogenous peripheral neurons
11
.
The CD271 antibody is reported to cross-react with the CD271 antigen in monkey, goat, dog, pig, and sheep
12
.

Columns

For positive selection: MS or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

Neural Crest Stem Cell MicroBeads, human

  • For research use only
for 1×
10
9
total cells
130-097-127
-