Pan-DC Enrichment Kit, human

Pan-DC Enrichment Kit, human

The Pan-DC Enrichment Kit is a fast pre-enrichment kit for untouched isolation of plasmacytoid and myeloid dendritic cells from PBMCs.

Detailed separation procedure

Using the Pan-DC Enrichment Kit, dendritic cells (MDC1s, MDC2s, and PDCs) are isolated very fast in a single step by depletion of non-target cells (negative selection). Non-target cells are labeled with a cocktail of biotin-conjugated monoclonal antibodies and Anti-Biotin MicroBeads. Additionally, they are directly magnetically labeled with a cocktail of MicroBead-conjugated antibodies against antigens that are not expressed by dendritic cells. The magnetically labeled non-target cells are depleted by retaining them within a MACS Column in the magnetic field of a MACS Separator while the unlabeled DCs run through.

Downstream applications

Dendritic cells pre-enriched with the Pan-DC Enrichment Kit can be used for:
  • Analysis of antigen uptake, processing, and presentation
  • Stimulation of primary T cell responses by antigen-pulsed dendritic cells
  • Analysis of gene expression in blood dendritic cells
  • Elucidation of specific signal transduction pathways

Columns

LS, XS, or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

Pan-DC Enrichment Kit, human

  • For research use only
for 2×
10
9
total cells
130-100-777
-

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