Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)

Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)

The IFN-γ Secretion Assays - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IFN-γ-secreting cells.

Background information

Interferon-gamma (IFN-γ) is predominantly secreted by activated CD8
+
and CD4
+
memory and effector T cells as well as by NK cells. It is mainly involved in the regulation of inflammatory immune responses. These T1 types of immune mechanisms are effective against intracellular pathogens and tumors and can be involved in immunological disorders, such as autoimmune reactions.

Downstream applications

The Mouse IFN-γ Secretion Assay was used to analyze the sequential production of cytokines by individual staphylococcal enterotoxin B-activated T helper lymphocytes
1
and for studies in T
H
1 cell differentiation
2,3
.
The Mouse IFN-γ Secretion Assay was also used for isolation of tumor-reactive T cells from immunized mice for adoptive T cell transfer experiments
4
and for analysis of T cells from mice immunized with viral antigens
5
. Murine NKT cells were analyzed
6,7,8
for IFN-γ secretion and isolated
7
after stimulation with glycolipid antigens. IFN-γ expression by T cells was also analyzed for intrahepatic mononuclear cells
9
and cells from bronchioalveolar lavages
10
. The Mouse IFN-γ Secretion Assay can also be combined with peptide-tetramer staining.
8

Columns

MS, LS, or autoMACS
®
Columns.
  • Selected References

    1. Assenmacher et al. (1998) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes. Eur. J. Immunol. 28: 1534-1543
    2. Wu, C. Y. et al. (2002) Distinct lineages of T(H)1 cells have differential capacities for memory cell generation in vivo. Nat. Immunol. 3: 852-858
    3. Zhang, Y. et al. (2001) Interferon gamma stabilizes the T helper cell type 1 phenotype. J. Exp. Med. 194: 165-172
    4. Becker, C. et al. (2001) Adoptive tumor therapy with T lymphocytes enriched through an IFN-gamma capture assay. Nat. Med. 7: 1159-1162
    5. Riberdy, J. M. et al. (2001) Cutting edge: culture with high doses of viral peptide induces previously unprimed CD8(+) T cells to produce cytokine. J. Immunol. 167: 2437-2440
    6. Hayakawa et al. (2001) Critical contribution of IFN-gamma and NK cells, but not perforin-mediated cytotoxicity, to anti-metastatic effect of alpha-galactosylceramide. Eur. J. Immunol. 31: 1720-1727
    7. Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153
    8. Morris, S. C. et al. (2006)
      IL-4 induces
      in vivo
      production of IFN-gamma by NK and NKT cells.
      J. Immunol. 176: 5299-5305
    9. Berenzon, D. et al. (2003)
      Protracted protection to
      Plasmodium berghei
      malaria is linked to functionally and phenotypically heterogeneous liver memory CD8
      +
      T cells.
      J. Immunol. 171: 2024-2034
    10. Mc Allister, F. et al. (2004)
      T cytotoxic-1 CD8
      +
      T cells are effector cells against pneumocystis in mice.
      J. Immunol. 172: 1132-1138
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Product information

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Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)

  • For research use only
for 50
tests
(1)
130-090-517
-