Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)

Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)

The IFN-γ Secretion Assays - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IFN-γ-secreting cells.

Background information

Interferon-gamma (IFN-γ) is predominantly secreted by activated CD8
+
and CD4
+
memory and effector T cells as well as by NK cells. It is mainly involved in the regulation of inflammatory immune responses. These T1 types of immune mechanisms are effective against intracellular pathogens and tumors and can be involved in immunological disorders, such as autoimmune reactions.

Downstream applications

The Mouse IFN-γ Secretion Assay was used to analyze the sequential production of cytokines by individual staphylococcal enterotoxin B-activated T helper lymphocytes
1
and for studies in T
H
1 cell differentiation
2,3
.
The Mouse IFN-γ Secretion Assay was also used for isolation of tumor-reactive T cells from immunized mice for adoptive T cell transfer experiments
4
and for analysis of T cells from mice immunized with viral antigens
5
. Murine NKT cells were analyzed
6,7,8
for IFN-γ secretion and isolated
7
after stimulation with glycolipid antigens. IFN-γ expression by T cells was also analyzed for intrahepatic mononuclear cells
9
and cells from bronchioalveolar lavages
10
. The Mouse IFN-γ Secretion Assay can also be combined with peptide-tetramer staining.
8

Columns

MS, LS, or autoMACS
®
Columns.
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