IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

The IL-4 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as enrichment of human IL-4-secreting cells.

Background information

Interleukin-4 (IL-4) is predominantly secreted by activated CD4
+
memory and effector T
H
2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T2 types of immune mechanisms can also be involved in immunologic disorders such as IgE-mediated allergy.

Downstream applications

IL-4 Secretion Assay was used to isolate and analyze Tetanus Toxoid-specific
1
, allergen-specific
2,5
, and autoreactive
7
T cells from PBMCs, as well as tumor-specific cells from T cell lines
3
. The IL-4 Secretion Assay was also used for isolation of IL-4-secreting human NK cells
4
, and for detection of IL-4-secreting NKT cells
6
.
IL-4-secreting CD8
+
T cells were isolated in combination with CD8 MultiSort MicroBeads.
8

Columns

MS, LS, or autoMACS
®
Columns.
Product options: 1

Product information

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Order no.
Price

IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

  • For research use only
for 50
tests
(1)
130-054-101
-

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