IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human

The IL-10 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-10-secreting cells.

Background information

Interleukin-10 (IL-10) is predominantly secreted by human CD4
+
T cells and antigen-presenting cells, such as monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by T
H
1 cells, activated monocytes, and NK cells. It can stimulate the immunoglobulin production by B cells.

Downstream applications

IL-10 Secretion Assays were used to analyze IL-10-secreting, CMV-specific CD4
+
T cells after stimulation with CMV lysate. The IL-10 Secretion Assay was also used to analyze IL-10 secretion by allergen-specific T cells.
1,2
Furthermore, it was used for the detection and isolation of antigen-specific IL-10-secreting type I regulatory T cells from patients with an autoimmune skin disease. The isolated cells were cloned, expanded and further analyzed for their regulatory function.
3

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MS, LS, or autoMACS
®
Columns.
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