IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE), human

IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE), human

The IFN-γ Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IFN-γ-secreting cells.
The used Anti-PE MicroBeads UltraPure enhances sensitivity and minimizes background of debris and dead cells.

Background information

Interferon gamma (IFN-γ) is predominantly secreted by activated CD8
+
and CD4
+
memory and effector T cells and by NK cells. It is mainly involved in the regulation of inflammatory immune responses. These T1 types of immune mechanisms are effective against intracellular pathogens and tumors. IFN-γ-secreting T cells can also be involved in immunological disorders such as autoimmune reactions.

Downstream applications

Virus-specific T cells were investigated after stimulation with peptides or proteins derived from influenza virus
1
, CMV
2,3
, EBV
4,5,6
, HIV
5,7,8,9,10
, HBV
11
, and ADV
24
.
Virus-specific T cells were expanded
in vitro1,2,3,5,6,9
showing highly specific and very efficient killing of target cells and have been analyzed for TCR clonotypes
8,10
.
IFN-γ Secretion Assays were used for the isolation and analysis of antigen-specific T cells from PBMCs after stimulation with Tetanus Toxoid
1
, minor histocompatibility antigens, and tumor antigens
12,13,14,15
. IFN-γ Secretion Assays were also used to purify and analyze tumor-specific T cells from T cell lines
13,14
and for isolation of functional antigen-specific, IFN-γ-secreting T cells reacting to other tumor antigens, e.g., SSX
14
, CEA
16
, or HER2
17
from PBMCs or TILs (tumor infiltrating lymphocytes)
14,18
.
The IFN-γ Secretion Assay was used to counterstain peptide-MHC-tetramer-labeled Melan A-specific CD8
+
T cells to analyze functionality of the tetramer-positive cells.
12,15
The IFN-γ Secretion Assay was also used for isolation and functional characterization of allergen-specific T cells.
21
Furthermore, the IFN-γ Secretion Assay was used for epitope mapping of MHC class II peptides.
19
IFN-γ-secreting human NK cells were isolated using the IFN-γ Secretion Assay.
20
IFN-γ Secretion Assay reagents were reported to cross-react with chimpanzee cells
22
, but not with rhesus macaque cells.
The IFN-γ Secretion Assays can also be used for two-color cytokine analysis
9
and allows counterstaining of peptide-MHC-tetramer-labeled cells
12,15
. It can also be combined with flow cytometric proliferation assays
23
.

Columns

MS, LS, or autoMACS
®
Columns.
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