Basic principle of MACS® MicroBead Technology

Flexible and gentle cell isolation with MACS MicroBeads

Benefit from our column-based cell isolation technology using nano-sized beads for positive cell selection or cell depletion of virtually any cell type from any cell source. 

MACS Technology using nano-sized beads

Benefit from our column-based technology using nano-sized beads for positive cell selection or cell depletion from any starting material, including peripheral blood mononuclear cells (PBMCs), whole blood, bone marrow, leukopak, and buffy coat. 

Minimal labeling

Minimal labeling preserves cellular integrity and characteristics.
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Flexible and gentle cell isolation method
MACS Column-based technology owes its longstanding success to the combination of superparamagnetic nano-sized beads and a very high magnetic gradient in our MACS Columns. This combination allows for minimal labeling of target cells and therefore preservation of cell integrity and characteristics. MACS MicroBeads are biodegradable and compatible with all downstream applications, including cell sorting, flow analysis, cell culture, functional assays and molecular analysis.

No epitope blocking or cell activation

MACS Technology minimizes epitope blocking and cell activation.
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Positive selection with MACS Technology minimizes unwanted effects on target cells
The high magnetic gradient generated by the matrix in the MACS Column allows for minimal labeling of target cells with nano-sized MicroBeads. This ensures that plenty of surface epitopes remain free for subsequent fluorescent staining and flow cytometry analysis.

Truly untouched cells

Depletion with MACS Technology ensures isolation of truly untouched target cells.
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Depletion of unwanted cells with MACS Technology: the only method to isolate truly untouched cells
Depletion of unwanted cells with MACS MicroBead Technology is an elegant way to obtain pure untouched target cells. Minimal labeling of  unwanted cells with MACS MicroBeads avoids non-specific labeling of target cells, leaving target cells truly untouched. In contrast, column-free methods using nano-sized beads require high concentrations of labeling reagents thus risking non-specific labeling of the target cell fraction.

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Brochure
MACS Cell Separation

Download our MACS Cell Separation brochure and learn more about MACS Technology cell isolation solutions.

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  • Siglec-5/Siglec-14
  • Siglec-8
  • Siglec-10
  • Siglec-F
  • Siglec-H
  • SIT1
  • Slan (M-DC8)
  • SLP-76
  • Slug
  • Sox1
  • Sox2
  • Sox17
  • Src pY418
  • SSEA-1
  • SSEA-4
  • SSEA-5
  • SSRP1
  • STAT1 N-terminus
  • STAT1 pS727
  • STAT1 pY701
  • STAT3 pS727
  • STAT4 pY693
  • STAT5a
  • STAT6
  • STAT6 pY641
  • Survivin
  • SUSD2
  • Syk
  • Syk pY348
  • Syndecan-4
  • T-bet
  • TCL1
  • TCR Vα2
  • TCR Vα3.2 (b, c)
  • TCR Vα7.2
  • TCR Vα11.1, 11.2
  • TCR Vα24
  • TCR Vβ1
  • TCR Vβ2
  • TCR Vβ3
  • TCR Vβ4
  • TCR Vβ5
  • TCR Vβ5.1
  • TCR Vβ5.3
  • TCR Vβ6
  • TCR Vβ7.1
  • TCR Vβ7.2
  • TCR Vβ8
  • TCR Vβ8.1, 8.2
  • TCR Vβ9
  • TCR Vβ10
  • TCR Vβ11
  • TCR Vβ12
  • TCR Vβ13.1
  • TCR Vβ13.6
  • TCR Vβ14
  • TCR Vβ16
  • TCR Vβ17
  • TCR Vβ17a
  • TCR Vβ21.3
  • TCR Vβ23
  • TCR Vγ9
  • TCR Vδ1
  • TCR Vδ2
  • TCR Vδ4
  • TCRα/β
  • TCRβ
  • TCRγ/δ
  • TdT
  • Ter-119
  • TGF-β RII
  • THEMIS
  • TIGIT
  • TIM-1
  • TIM-3
  • TIM-4
  • TIMP-3
  • TLT-2
  • TM4SF1
  • TNF-α
  • TNFRH3
  • TOM22
  • TOX
  • TRA-1-60
  • TRA-1-81
  • TRA-1-85 (CD147)
  • TrkA
  • TROP2
  • TSPAN8
  • TTF-1
  • Under-phospho Rb
  • VEGFR-1
  • Vimentin
  • WIP pS488
  • XCR1
  • ZAP70
  • ZAP70 pY292
  • α-Actinin
  • β-Catenin
  • 1A5
  • 1A8
  • 1B1
  • 1C9-2
  • 1G7.G10
  • 2.29
  • 2A2
  • 2A9-1
  • 2C9.G2 (HMβ3-1)
  • 2D10
  • 2E1
  • 3B2/TA8
  • 3C11
  • 3C12
  • 3G3
  • 3G10
  • 4B4-1
  • 4B12
  • 4D11
  • 4E3
  • 4E5
  • 4FR6D3
  • 5-329
  • 5.133
  • 5B1
  • 5C8
  • 5D3
  • 5E8.4
  • 5H10-27
  • 6B11
  • 6D4
  • 6D5
  • 7-239
  • 7A3-3
  • 7C9
  • 7D4
  • 7E.17G9
  • 7H22-E16
  • 8e11
  • 8F1
  • 8F9
  • 8F10
  • 9E2
  • 10.1.1
  • 10D12
  • 11-26c.2a
  • 11C1
  • 11F2
  • 11F8
  • 11PB6
  • 12G5
  • 13G3
  • 14B11
  • 15E8
  • 16-10A1
  • 17A2
  • 17B5-1H1
  • 18d3
  • 19-3
  • 20/70
  • 20d5
  • 22F6
  • 23C6
  • 24D2
  • 24F
  • 25-D1.16
  • 29A1.4.9
  • 30-H12
  • 30F11
  • 32D12
  • 43A4E1
  • 44F9
  • 45-15
  • 53-6.7
  • 53-7.3
  • 67A4
  • 67D2
  • 87G
  • 90.4
  • 93
  • 97C5
  • 104-2
  • 105HB29
  • 123R3
  • 145-2C11
  • 145/15
  • 187.1
  • 188B
  • 191B8
  • 293C3
  • 390
  • 429 (MVCAM.A)
  • 541-10B2
  • 551.3D3
  • 555
  • A1
  • A3C6E2
  • A7R 34
  • A20
  • AC102
  • AC104.3E3
  • AC106
  • AC122
  • AC128
  • AC133
  • AC136
  • AC144
  • AC145
  • ACSA-1
  • ACT35
  • AD2
  • AD5-8E7
  • AD5-14H12
  • AD5-17F6
  • AF12-7H3
  • AF29-4D12
  • APA5
  • APB5
  • ATM-2
  • Avas12
  • B3B4
  • B4B6
  • BAT221
  • BB27
  • Ber-ACT 8
  • Bio3-18E7
  • BT3.1
  • BV10A4H2
  • BVD4-1D11
  • BW135/80
  • BW242/412
  • BW264/56
  • C9B7W
  • C363-16A
  • cA2
  • caa7-9G8
  • CD7-6B7
  • CH3A4A7
  • CH6-17E5.1
  • Cr24.1
  • CRA1
  • CW-1.2
  • CX5
  • Cy34.1
  • CZ8-23G1
  • D7
  • D7715A7
  • DA03-22H2.8
  • DA04-10E8.9
  • DB105
  • DCN47.5
  • DCN228
  • DD-1
  • DF-T1
  • DG3
  • DJR1
  • DJR3
  • DN254
  • DT5D3
  • DX2
  • DX5
  • DX9
  • DX11
  • DX27
  • E11
  • EAT2
  • EP-1
  • ES8-20E6
  • ES22-10D8
  • ES26-5E12.4
  • ES26-14D1.11
  • ES26-15B7.3
  • F38-2E2
  • FA-11
  • FGK45.5
  • FM95
  • FM276
  • FN50
  • FR3-16A11
  • FR10-11G9
  • FR 70 
  • GHI/61.1
  • GHI/75
  • GK1.5
  • GL3
  • GoH3
  • H1.2F3
  • H4A3
  • H4B4
  • H5C6
  • H35-17.2
  • H44
  • H100-27.R55
  • H194-112
  • HA2-7B1
  • HB5
  • HB14
  • HB15
  • HBF-719
  • hC3aRZ8
  • HD30
  • HEA-125
  • HECA-452
  • HI149
  • His51
  • HK5.3
  • Hly9.1.25
  • HM48-1
  • HM79-12
  • HMD4-1
  • HMN2-35
  • HMß1-1
  • HMα5-1
  • HSL96
  • HTF-1
  • IB6
  • IgD26
  • IM7.8.1
  • IS5-20C4
  • IS5-21F5
  • IS6-11E5.11
  • IS7-24C7
  • IS11-3B2.2.3
  • IS11-8E10
  • IS11-21E11
  • IS11-24D5
  • ITEM-4
  • JC5-1
  • JD3
  • JES1-39D10
  • JES3-9D7
  • JES5-16E3
  • JF05-1C2.4.1
  • JJC11.6
  • JS11
  • KJ1-26
  • L11
  • LG.3A10
  • LN2-9C4
  • LT2
  • LT19
  • LT20
  • LT27:295
  • M-L23.4
  • M-T271
  • M-T411
  • M-T466
  • M1/69
  • M1/70.15.11.5
  • M3/38
  • M3/84
  • M5/114.15.2
  • M18/2
  • MAR-1
  • MB4-6D6
  • MB9-3G8
  • MB10-5C4
  • MB15-18C9
  • MB22-9F5
  • MD5-1-3C2
  • ME-9F1
  • ME20.4-1.H4
  • MEL14-H2.100
  • MFL3
  • MHD1-314
  • MHD4-46
  • MHR73-11
  • MIH12
  • MIH18
  • MIH37
  • MJ4-27G12
  • MJ7/18
  • MQ2-13A5
  • MRα4-1
  • MWReg30
  • MZ3
  • MZ18-23C8
  • MZ18-24A9
  • N7.48 A
  • N418
  • NKVFS1
  • NLDC-145
  • NOK-1
  • O4
  • OX-104
  • PD1.3.1.3
  • PJ2-22H3
  • PK136
  • PO3.3
  • PV404.19
  • R1-2
  • R1.302
  • RA3-6B2
  • RB6-8C5
  • REA100
  • REA101
  • REA102
  • REA103
  • REA104
  • REA107
  • REA108
  • REA109
  • REA110
  • REA111
  • REA112
  • REA113
  • REA114
  • REA115
  • REA118
  • REA119
  • REA120
  • REA121
  • REA122
  • REA124
  • REA125
  • REA126
  • REA127
  • REA128
  • REA129
  • REA134
  • REA138
  • REA140
  • REA141
  • REA142
  • REA143
  • REA144
  • REA145
  • REA146
  • REA147
  • REA148
  • REA151
  • REA153
  • REA154
  • REA158
  • REA161
  • REA163
  • REA164
  • REA165
  • REA167
  • REA168
  • REA170
  • REA171
  • REA173
  • REA174
  • REA175
  • REA176
  • REA179
  • REA181
  • REA183
  • REA185
  • REA187
  • REA188
  • REA190
  • REA191
  • REA192
  • REA193
  • REA194
  • REA195
  • REA196
  • REA198
  • REA199
  • REA200
  • REA202
  • REA203
  • REA204
  • REA205
  • REA206
  • REA207
  • REA208
  • REA209
  • REA211
  • REA213
  • REA214
  • REA215
  • REA217
  • REA218
  • REA219
  • REA220
  • REA221
  • REA222
  • REA223
  • REA226
  • REA227
  • REA228
  • REA230
  • REA231
  • REA232
  • REA233
  • REA234
  • REA236
  • REA237
  • REA238
  • REA241
  • REA244
  • REA245
  • REA247
  • REA250
  • REA252
  • REA254
  • REA256
  • REA257
  • REA258
  • REA259
  • REA260
  • REA261
  • REA263
  • REA264
  • REA265
  • REA266
  • REA269
  • REA273
  • REA274
  • REA276
  • REA277
  • REA278
  • REA280
  • REA282
  • REA284
  • REA286
  • REA287
  • REA289
  • REA290
  • REA291
  • REA292
  • REA293
  • REA296
  • REA298
  • REA299
  • REA300
  • REA301
  • REA302
  • REA303
  • REA304
  • REA306
  • REA308
  • REA309
  • REA311
  • REA312
  • REA313
  • REA314
  • REA316
  • REA318
  • REA319
  • REA321
  • REA322
  • REA325
  • REA327
  • REA329
  • REA332
  • REA336
  • REA337
  • REA339
  • REA340
  • REA343
  • REA344
  • REA347
  • REA349
  • REA351
  • REA354
  • REA359
  • REA360
  • REA361
  • REA363
  • REA364
  • REA366
  • REA368
  • REA372
  • REA374
  • REA377
  • REA378
  • REA381
  • REA382
  • REA384
  • REA386
  • REA387
  • REA388
  • REA389
  • REA391
  • REA392
  • REA393
  • REA395
  • REA396
  • REA398
  • REA400
  • REA401
  • REA402
  • REA405
  • REA408
  • REA410
  • REA412
  • REA413
  • REA414
  • REA419
  • REA421
  • REA422
  • REA423
  • REA425
  • REA426
  • REA428
  • REA429
  • REA431
  • REA437
  • REA440
  • REA441
  • REA442
  • REA443
  • REA444
  • REA445
  • REA446
  • REA447
  • REA448
  • REA450
  • REA452
  • REA453
  • REA456
  • REA460
  • REA461
  • REA464
  • REA465
  • REA466
  • REA467
  • REA469
  • REA470
  • REA471
  • REA472
  • REA476
  • REA478
  • REA479
  • REA482
  • REA484
  • REA489
  • REA490
  • REA492
  • REA493
  • REA494
  • REA495
  • REA496
  • REA497
  • REA499
  • REA501
  • REA503
  • REA504
  • REA505
  • REA508
  • REA509
  • REA510
  • REA512
  • REA513
  • REA517
  • REA518
  • REA519
  • REA523
  • REA524
  • REA526
  • REA527
  • REA528
  • REA531
  • REA532
  • REA535
  • REA538
  • REA540
  • REA541
  • REA542
  • REA543
  • REA545
  • REA546
  • REA547
  • REA550
  • REA553
  • REA554
  • REA557
  • REA558
  • REA559
  • REA560
  • REA561
  • REA562
  • REA563
  • REA564
  • REA566
  • REA568
  • REA570
  • REA571
  • REA572
  • REA573
  • REA574
  • REA577
  • REA578
  • REA581
  • REA585
  • REA587
  • REA589
  • REA591
  • REA592
  • REA594
  • REA596
  • REA598
  • REA599
  • REA600
  • REA601
  • REA602
  • REA603
  • REA604
  • REA605
  • REA606
  • REA607
  • REA608
  • REA610
  • REA611
  • REA612
  • REA613
  • REA614
  • REA616
  • REA617
  • REA618
  • REA619
  • REA620
  • REA621
  • REA623
  • REA624
  • REA625
  • REA627
  • REA629
  • REA630
  • REA631
  • REA633
  • REA634
  • REA635
  • REA637
  • REA639
  • REA640
  • REA641
  • REA642
  • REA645
  • REA646
  • REA648
  • REA649
  • REA651
  • REA652
  • REA654
  • REA657
  • REA662
  • REA663
  • REA664
  • REA665
  • REA669
  • REA670
  • REA671
  • REA672
  • REA674
  • REA675
  • REA677
  • REA679
  • REA680
  • REA683
  • REA684
  • REA686
  • REA687
  • REA688
  • REA689
  • REA690
  • REA692
  • REA693
  • REA694
  • REA695
  • REA699
  • REA701
  • REA706
  • REA707
  • REA709
  • REA711
  • REA713
  • REA714
  • REA715
  • REA717
  • REA718
  • REA722
  • REA723
  • REA724
  • REA727
  • REA729
  • REA730
  • REA733
  • REA734
  • REA736
  • REA737
  • REA738
  • REA739
  • REA740
  • REA741
  • REA743
  • REA744
  • REA745
  • REA746
  • REA747
  • REA748
  • REA749
  • REA750
  • REA752
  • REA753
  • REA754
  • REA755
  • REA757
  • REA758
  • REA759
  • REA760
  • REA761
  • REA762
  • REA764
  • REA765
  • REA768
  • REA769
  • REA770
  • REA771
  • REA772
  • REA773
  • REA774
  • REA775
  • REA776
  • REA777
  • REA778
  • REA779
  • REA780
  • REA781
  • REA782
  • REA784
  • REA785
  • REA786
  • REA787
  • REA788
  • REA789
  • REA790
  • REA791
  • REA792
  • REA793
  • REA794
  • REA795
  • REA796
  • REA797
  • REA798
  • REA800
  • REA802
  • REA803
  • REA804
  • REA805
  • REA807
  • REA808
  • REA809
  • REA810
  • REA811
  • REA812
  • REA813
  • REA815
  • REA816
  • REA817
  • REA818
  • REA819
  • REA820
  • REA822
  • REA823
  • REA824
  • REA826
  • REA827
  • REA828
  • REA829
  • REA831
  • REA832
  • REA833
  • REA834
  • REA835
  • REA836
  • REA838
  • REA839
  • REA840
  • REA841
  • REA843
  • REA844
  • REA845
  • REA846
  • REA847
  • REA849
  • REA850
  • REA851
  • REA852
  • REA853
  • REA854
  • REA855
  • REA856
  • REA857
  • REA858
  • REA859
  • REA860
  • REA861
  • REA863
  • REA864
  • REA865
  • REA867
  • REA869
  • REA870
  • REA871
  • REA874
  • REA875
  • REA876
  • REA877
  • REA878
  • REA879
  • REA880
  • REA882
  • REA884
  • REA886
  • REA889
  • REA890
  • REA892
  • REA893
  • REA894
  • REA897
  • REA898
  • REA899
  • REA901
  • REA902
  • REA903
  • REA905
  • REA906
  • REA907
  • REA909
  • REA910
  • REA911
  • REA912
  • REA915
  • REA916
  • REA918
  • REA923
  • REA932
  • REA934
  • REA936
  • REA937
  • REA940
  • REA941
  • REA942
  • REA943
  • REA945
  • REA946
  • REA947
  • REA948
  • REA950
  • REA951
  • REA953
  • REA954
  • REA955
  • REA956
  • REA957
  • REA958
  • REA959
  • REA960
  • REA961
  • REA962
  • REA963
  • REA964
  • REA965
  • REA967
  • REA968
  • REA969
  • REA970
  • REA971
  • REA972
  • REA975
  • REA976
  • REA977
  • REA978
  • REA979
  • REA980
  • REA981
  • REA983
  • REA984
  • REA985
  • REA988
  • REA989
  • REA990
  • REA991
  • REA994
  • REA996
  • REA998
  • REA999
  • REA1000
  • REA1002
  • REA1003
  • REA1005
  • REA1006
  • REA1007
  • REA1008
  • REA1010
  • REA1011
  • REA1012
  • REA1013
  • REA1014
  • REA1015
  • REA1016
  • REA1017
  • REA1018
  • REA1021
  • REA1023
  • REA1025
  • REA1026
  • REA1028
  • REA1030
  • REA1031
  • REA1034
  • REA1036
  • REA1037
  • REA1039
  • REA1040
  • REA1041
  • REA1042
  • REA1043
  • REA1045
  • REA1046
  • REA1047
  • REA1048
  • REA1049
  • REA1051
  • REA1053
  • REA1054
  • REA1055
  • REA1056
  • REA1057
  • REA1058
  • REA1061
  • REA1062
  • REA1065
  • REA1066
  • REA1067
  • REA1070
  • REA1071
  • REA1072
  • REA1075
  • REA1076
  • REA1077
  • REA1078
  • REA1080
  • REA1081
  • REA1083
  • REA1085
  • REA1090
  • REA1094
  • REA1095
  • REA1096
  • REA1097
  • REA1098
  • REA1100
  • REA1103
  • REA1105
  • REA1106
  • REA1110
  • REA1111
  • REA1115
  • REA1116
  • REA1129
  • REA1133
  • RG7/1.30
  • RG7/11.1
  • RG11/39.2
  • RM2-5
  • RM134L
  • RMT1-17
  • RP/14
  • S5/1
  • S43.10
  • SN4 C3-3A2
  • SN13
  • T6D11
  • TB03
  • TC11-18H10
  • Ter-119
  • TET2
  • TKS-1
  • TLR 3.7
  • TM-β1
  • TS1/18
  • TS2/7
  • TS2/9
  • TS2/16
  • TY/11.8
  • TÜK4
  • UCHL1
  • UCHT2 
  • UP-H2
  • UP-R1
  • VIM3b
  • VIM5
  • VIM8
  • VIMC6
  • VIMD2
  • VIT4
  • W5C5
  • X-54
  • X-56
  • X-57
  • Y1/82A
  • Y2/51
  • YLI-90
  • goat IgG
  • hamster IgG
  • hamster IgG1
  • hamster IgG1κ
  • hamster IgG1λ
  • hamster IgG2
  • hamster IgG2κ
  • hamster IgG2λ
  • hamster IgG3κ
  • hamster IgGκ
  • hamster IgGλ
  • human IgG1
  • human IgG2
  • mouse IgG1
  • mouse IgG1κ
  • mouse IgG2a
  • mouse IgG2aκ
  • mouse IgG2b
  • mouse IgG2bκ
  • mouse IgG3κ
  • mouse IgM
  • mouse IgMκ
  • rabbit IgG
  • rat IgG1
  • rat IgG1κ
  • rat IgG2a
  • rat IgG2aκ
  • rat IgG2aλ
  • rat IgG2b
  • rat IgG2bκ
  • rat IgG2bλ
  • rat IgG2c
  • rat IgMκ
  • recombinant human IgG1
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  • Sample preparation
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  • Western blotting
  • Alkaline Phos­phatase
  • APC
  • APC-Vio770
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  • FITC
  • HRP
  • HRP (C.-term.)
  • PE
  • PE-Vio615
  • PE-Vio770
  • PerCP
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  • pure
  • Vio488
  • Vio515
  • Vio667
  • VioBlue
  • VioBright 515
  • VioBright 667
  • VioBright FITC
  • VioGreen
  • Blue 488 nm
  • Green 532 nm
  • other
  • Red 635 nm
  • UV
  • Violet 405 nm
  • Yellow 550 nm
  • Yellow 561 nm
  • Sample dissociation
  • Sample mixing
  • Activin A
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  • BAFF
  • BD-2
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  • BDNF
  • BMP-2
  • BMP-4
  • BMP-6
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  • EGF
  • Exodus 2
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  • GDF-11
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  • IL-15
  • IL-15Rα sushi
  • IL-17A
  • IL-17F
  • IL-19
  • IL-21
  • IL-22
  • IL-23
  • IL-24
  • IL-25
  • IL-27
  • IL-31
  • IL-33
  • IL-34
  • IL-35
  • IP-10
  • LIF
  • M-CSF
  • MCP-1
  • MIF
  • MIG
  • MIP-3a
  • NGF-β
  • Noggin
  • NT-3
  • NT-4
  • Oncostatin M
  • OPG
  • PDGF-AA
  • PDGF-AB
  • PDGF-BB
  • Pleiotrophin
  • Prolactin
  • R-Spondin-2
  • R-Spondin-3
  • RANK-Ligand
  • RANTES
  • SCF
  • SDF-1α
  • SHH (C24II)
  • TARC
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  • TGF-β3
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  • Pichia pastoris
  • Yeast
  • Cell line–specific media
  • Immune cell media
  • Media for neural cells
  • Stem cell media
  • Functional-grade antibodies
  • MACSiBead Particle–based
  • Oligonucleotides
  • other
  • Peptide pools
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  • TGFβ/Smad
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  • cDNA synthesis
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  • Protein isolation
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  • Optical imaging
  • Ultrasound
  • Gadolinium
  • Indocyanine green
  • Iodine
  • Microbubbles
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  • SPIO
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