Switched Memory B Cell Isolation Kit, human

Switched Memory B Cell Isolation Kit, human

The Switched Memory B Cell Isolation Kit was developed for the fast and efficient isolation of untouched switched memory B cells from human PBMCs.

Background information

Switched Memory B cells are indicators of normal B cell activation and development in germinal centers of lymph nodes or other secondary lymphoid organs. The amount of switched memory B cells in peripheral blood is an indicator for humoral immunodeficiency, such as specific antibody deficiency (SAD) or common variable immunodeficiency (CVID).

Detailed separation procedure

Non-target cells are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, CD235a (glycophorin A), Anti-IgM, and Anti-IgD antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Highly pure switched memory B cells are obtained by depletion of the magnetically labeled cells.

Downstream applications

Purified switched memory B cells can be used for studies in cell biology or molecular biology and functional assays.

Columns

LS or autoMACS
®
Columns.
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Product information

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Switched Memory B Cell Isolation Kit, human

  • For research use only
for 2×
10
9
total cells
130-093-617
-

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