MZ and FO B Cell Isolation Kit, mouse

MZ and FO B Cell Isolation Kit, mouse

The Marginal Zone and Follicular B Cell Isolation Kit has been developed for the isolation of marginal zone (MZ) B cells and follicular (FO) B cells from mouse spleen.

Background information

The isolation of CD21
hi
CD23
lo/–
marginal zone (MZ) and CD21
int
CD23
hi
follicular (FO) B cells is performed in a two-step procedure. First, the non-B cells, the B-1 cells, and the transitional B cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies, as primary labeling reagent, and Anti-Biotin MicroBeads, as secondary labeling reagent. In between the two labeling steps no washing steps are required. The labeled cells are subsequently depleted by separation over a MACS
®
Column, which is placed in the magnetic field of a MACS Separator. The unlabeled fraction contains the pre-enriched MZ and FO B cells. In order to further separate these two subsets, the FO B cells are directly labeled with CD23 MicroBeads in a second step and isolated by separation over a MACS Column, which is placed in the magnetic field of a MACS Separator. The flow-through contains the enriched MZ B cells. After removing the column from the magnetic field, the magnetically retained FO B cells can be eluted as the positively selected cell fraction.

Columns

LS or autoMACS
®
Columns.
Product options: 1

Product information

Size
Order no.
Price

MZ and FO B Cell Isolation Kit, mouse

  • For research use only
for 2×
10
9
total cells
130-100-366
-

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