Germinal Center B Cell (PNA) MicroBead Kit, mouse

Germinal Center B Cell (PNA) MicroBead Kit, mouse

B cells within germinal centers express ligands for peanut agglutinin (PNA), allowing for their identification and isolation.
The Germinal Center B Cell (PNA) MicroBead Kit, mouse has been optimized for the rapid and simple isolation of mouse germinal center B cells from single-cell suspensions from lymphoid tissues upon immunization.

Background information

Following primary immunization with T-dependent antigen, B cells in the lymphatic tissues can form germinal centers (GC). Here B cells proliferate, differentiate, and mutate their antibody genes through somatic hypermutation, and switch the class of their antibody.
B cells within GC express ligands for peanut agglutinin (PNA), allowing for their identification and isolation. PNA is a plant lectin protein derived from the fruits of
Arachis hypogae
, which specifically binds to terminal non-reducing galactose residues on the cellular membrane.

Downstream applications

Germinal center B cell isolated with Germinal Center B Cell (PNA) MicroBead Kit, mouse can be used for phenotypical and functional analyses of germinal center B cells, e.g. antibody screening, cytokine secretion, antigen presentation and cell development studies in different experimental murine models of immunization.

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MS or LS Columns.
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