CD45R (B220) MicroBeads, mouse

CD45R (B220) MicroBeads, mouse

Mouse CD45R (B220) MicroBeads were developed for the positive selection or depletion of mouse B cells from various lymphoid tissues like bone marrow, lymph nodes, and spleen or from non-lymphoid sources like lamina propria, peritoneal cavity, or lung.

Background information

The CD45R antigen is expressed on B lineage cells throughout their development from early pro-B cell stages on and is down-regulated upon terminal differentiation to plasma cells. CD45R is also expressed on plasmacytoid dendritic cells.

Downstream applications

B cells purified with CD45R MicroBeads have been used for studies on the mechanism and regulation of immunoglobulin gene rearrangement,
1
for analyses of signal transduction,
2,3
and for reconstitution of immunodeficient and parasite-infected mice.
4

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected References

    1. Maki, K. et al. (2000) Immunoglobulin beta signaling regulates locus accessibility for ordered immunoglobulin gene rearrangements. J. Exp. Med. 191: 1333-1340
    2. Weintraub, B. C. et al. (2000) Entry of B cell receptor into signaling domains is inhibited in tolerant B cells. J. Exp. Med. 191: 1443-1448
    3. Suzuki, H. et al. (1999) Xid-like immunodeficiency in mice with disruption of the p85alpha subunit of phosphoinositide 3-kinase. Science 283: 390-392
    4. Hoerauf, A. et al. (1996)
      Co-transfer of B cells converts resistance into susceptibility in T cell-reconstituted,
      Leishmania major
      -resistant C.B-17 scid mice by a non-cognate mechanism.
      Int. Immunol. 8: 1569-1575
Product options: 2

Product information

Size
Order no.
Price

CD45R (B220) MicroBeads, mouse

  • For research use only
for 2×
10
9
total cells
130-049-501
-

CD45R (B220) MicroBeads, mouse - lyophilized

  • For research use only
for 2×
10
9
total cells
130-097-152
-