Anti-PE MicroBeads

Anti-PE MicroBeads

Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed. Anti-PE MicroBeads UltraPure have been especially designed for cell separations from debris-rich biological starting materials.

Background information

Anti-PE MicroBeads UltraPure have been especially developed for highly efficient separation of cells from debris-rich samples or other biological materials according to surface markers labeled with PE-conjugated primary antibodies, peptides or ligands. After separation the PE-labeled cells can be directly detected by flow cytometry or fluorescence microscopy without any further staining. Together with its ultrapure cell separation result even from debris-rich starting material Anti-PE MicroBeads UltraPure are the latest development of Anti-PE MicroBeads.

Applications

Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers
1,2
or MHC Class II tetramers
3
. Moreover, they have been used to separate a CD62L
+
subset of CD4
+
CD45RO
+
memory T cells from human adenoids, which had been pre-sorted by MACS® Technology
4
. The latest development of Anti-PE MicroBeads UltraPure enables high-yield positive cell separation using any PE-labeled primary antibody, even from low quality and rare cell samples.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected References

    1. Shankar et al. (2001) Blood 96: 3094-3101
    2. Barnes et al. (2004)
      Ultra-sensitive class I tetramer analysis reveals previously undetectable populations of antiviral CD8
      +
      T cells.
      Eur. J. Immunol. 34: 1570-1577
    3. Lucas, M. et al. (2004) Ex vivo phenotype and frequency of influenza virus-specific CD4 memory T cells. J. Virol. 78: 7284-7287
    4. Mattila, P. S. et al. (2000)
      Adenoids provide a microenvironment for the generation of CD4
      +
      , CD45RO
      +
      , L-selectin
      , CXCR4
      +
      , CCR5
      +
      T lymphocytes, a lymphocyte phenotype found in the middle ear effusion.
      Int. Immunol. 12: 1235-1243
Product options: 3

Product information

Size
Order no.
Price

Anti-PE MicroBeads

  • For research use only
for 1×
10
9
total cells
130-048-801
-

Anti-PE MicroBeads – lyophilized

  • For research use only
for 1×
10
9
total cells
130-097-054
-

Anti-PE MicroBeads UltraPure

  • For research use only
for 1×
10
9
total cells
130-105-639
-