Anti-FITC MicroBeads

Anti-FITC MicroBeads

Anti-FITC MicroBeads are widely used for indirect magnetic labeling and separation of cells or other materials which are labeled with a primary antibody or ligand conjugated to fluorescein isothiocyanate (FITC).

Background information

The FITC molecule is recognized by the monoclonal anti-FITC antibody coupled to MicroBeads, allowing the positive selection or depletion of the fluorescently labeled material with MACS
®
Technology. Labeling samples with FITC-conjugated primary antibodies and Anti-FITC MicroBeads enables subsequent flow cytometric analysis of separated cells without any further staining of the material.

Applications

Examples among the many separations performed with Anti-FITC MicroBeads are the isolation of IgD
+
B cells from human PBMCs
1
, the separation of plant protoplasts
2
as well as the isolation of murine Langerhans cells from epidermal tissue
3
. Anti-FITC MicroBeads have also been used for the depletion of CD3
+
cells from rat spleen and subsequent isolation of CD3
-
NK cells
5
or the separation of murine naive CD8
+
T cells expressing low levels of CD44 by depletion of CD44
int/high
cells
6
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
Product options: 2

Product information

Size
Order no.
Price

Anti-FITC MicroBeads

  • For research use only
for 2×
10
9
total cells
130-048-701
-

Anti-FITC MicroBeads – lyophilized

  • For research use only
for 2×
10
9
total cells
130-097-050
-