Anti-FITC MicroBeads

Anti-FITC MicroBeads

Anti-FITC MicroBeads are widely used for indirect magnetic labeling and separation of cells or other materials which are labeled with a primary antibody or ligand conjugated to fluorescein isothiocyanate (FITC).

Background information

The FITC molecule is recognized by the monoclonal anti-FITC antibody coupled to MicroBeads, allowing the positive selection or depletion of the fluorescently labeled material with MACS
®
Technology. Labeling samples with FITC-conjugated primary antibodies and Anti-FITC MicroBeads enables subsequent flow cytometric analysis of separated cells without any further staining of the material.

Applications

Examples among the many separations performed with Anti-FITC MicroBeads are the isolation of IgD
+
B cells from human PBMCs
1
, the separation of plant protoplasts
2
as well as the isolation of murine Langerhans cells from epidermal tissue
3
. Anti-FITC MicroBeads have also been used for the depletion of CD3
+
cells from rat spleen and subsequent isolation of CD3
-
NK cells
5
or the separation of murine naive CD8
+
T cells expressing low levels of CD44 by depletion of CD44
int/high
cells
6
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Zan, H. et al. (1998) CD40 engagement triggers switching to IgA1 and IgA2 in human B cells through induction of endogenous TGF-beta: evidence for TGF-beta but not IL-10-dependent direct S mu-->S alpha and sequential S mu-->S gamma, S gamma-->S alpha DNA recombination. J. Immunol. 161: 5217-5225
    2. Barth, S. et al. (1994) Selection and enrichment of differentially labeled plant protoplasts. J. Biochem. Biophys. Methods 29: 83-86
    3. Saeki, H. et al. (2000)
      A migratory population of skin-derived dendritic cells expresses CXCR5, responds to B lymphocyte chemoattractant
      in vitro
      , and co-localizes to B cell zones in lymph nodes
      in vivo
      .
      Eur. J. Immunol. 30(10): 2808-2814
    4. Kuypers, F. A. et al. (1996) Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V. Blood 87: 1179-1187
    5. Mikus, L.D. et al. (2001) Reduced interferon-gamma secretion by natural killer cells from rats susceptible to postviral chronic airway dysfunction. Am. J. Respir. Cell Mol. Biol. 24: 74-82
    6. Schüler, T. et al. (2004) Cutting edge: IL-7-dependent homeostatic proliferation of CD8+ T cells in neonatal mice allows the generation of long-lived natural memory T cells. J. Immunol. 172: 15-19
Product options: 2
130-048-701

Anti-FITC MicroBeads

for 2×
10
9
total cells
-
130-097-050

Anti-FITC MicroBeads – lyophilized

for 2×
10
9
total cells
-