ODN 2395

ODN 2395

Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines.
ODN 2395 is a C-class ODN for activation of B cells and pDCs.

Background information

TLR9 is a prominent member of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns. TLR9 recognizes specifically unmethylated CpG motifs in bacterial DNA leading to activation of immune cells
1
,
2
. These effects can be mimicked by short synthetical oligodeoxyribonucleotides (ODNs) containing unmethylated CpG motifs
3
.
Several classes of CpG ODN have been identified and can be distinguished by their effects on certain cell types. A-class ODNs containing 5’ and 3’ G-rich stretches induce high levels of type I IFN but show low activation of B cell proliferation. B-class ODNs activate B cells and TLR9-dependent NFkB signalling in recombinant cell lines but show low induction of IFN-α. C-class ODN induce high amounts of IFN-α and activate B cells. The recently discovered new P-Class ODNs show similar but superior properties to C-class ODNs.

Applications

C-class ODN for activation of B cells and pDCs and induction of IFN-α secretion.
C-Class ODN form dimeric structures.
Product options: 2

Product information

Size
Order no.
Price

ODN 2395

  • For research use only
200 µg
130-100-282
-

ODN 2395

  • For research use only
1 mg
130-100-283
-

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1 products available