ODN 1826

ODN 1826

Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines.
ODN 1826 strongly activates murine TLR9. It is a B-class ODN for activation of B cells, induction of Il-6 secretion, and activation of NF-kB-signalling pathways.

Background information

TLR9 is a prominent member of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns. TLR9 recognizes specifically unmethylated CpG motifs in bacterial DNA leading to activation of immune cells
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,
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. These effects can be mimicked by short synthetical oligodeoxyribonucleotides (ODNs) containing unmethylated CpG motifs
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.
Several classes of CpG ODN have been identified and can be distinguished by their effects on certain cell types. A-class ODNs containing 5’ and 3’ G-rich stretches induce high levels of type I IFN but show low activation of B cell proliferation. B-class ODNs activate B cells and TLR9-dependent NFkB signalling in recombinant cell lines but show low induction of IFN-α. C-class ODN induce high amounts of IFN-α and activate B cells. The recently discovered new P-Class ODNs show similar but superior properties to C-class ODNs.
ODN 1826 is a B-class ODN that strongly activates murine TLR9.

Applications

B-class ODN for activation of B cells , induction of Il-6 secretion, and activation of NF-kB-signalling pathways.
Murine B-Class ODN 1826 can be used as an adjuvant for vaccination strategies.
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Product options: 2

Product information

Size
Order no.
Price

ODN 1826

  • For research use only
200 µg
130-100-274
-

ODN 1826

  • For research use only
1 mg
130-100-103
-

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