ORN R-0002

ORN R-0002

TLR7 and TLR8 are members of the Toll-like-receptor (TLR) family, recognizing (virally derived) double- or single-stranded RNA, leading to activation of immune cells.
ORN R-0002 activates specifically human TLR8 and induces a TLR8-biased spectrum of cytokines from human PBMCs (predominantly TNF-α, IFN-γ, IL-12).
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Background information

TLR7 and TLR8 are members of the Toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns.
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The natural ligands of TLR7 and TLR8, double- or single-stranded RNA, can be mimicked by short synthetic oligoribonucleotides (ORNs).
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RNA motifs activating either TLR7 or TLR8 only or both, TLR7 and TLR8, have been described.
In human cells, TLR7 is predominantly expressed in pDCs and B cells, whereas TLR8 is mainly expressed on cells of the myeloid lineage, such as monocytes, macrophages, and myeloid dendritic cells (mDCs). In mice, TLR7 is also expressed on mDCs and monocytes. Murine TLR8 is functionally impaired.
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Due to the sensitive nature of RNA, most nucleic acid TLR7/8 agonists need to be formulated with cationic lipids in order to activate an appropiate immune response. The recommended cationic lipid is DOTAP-Cl (1,2-Dioleoyloxy-3-trimethylammonium-propane chloride).

Applications

TLR7 and TLR8 agonists can be used for the activation of immune cells, such as human PBMCs, murine splenocytes, or isolated immune cells, e.g., B cells, pDCs, mDCs, and monocytes.
ORN R-0002 specifically activates human TLR8.
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Product information

Size
Order no.
Price

ORN R-0002

  • For research use only
200 µg
130-104-427
-

ORN R-0002

  • For research use only
1 mg
130-104-438
-

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