Treg Expansion Kit, human

Treg Expansion Kit, human

The Treg Expansion Kit was developed for the efficient expansion of human Treg cells.

Background information

Using this kit FoxP3 expression by Treg cells is sustained after isolation with the CD4
+
CD25
+
Regulatory T cell Isolation Kit, CD4
+
CD25
+
CD127
dim/–
or CD4
+
CD25
+
CD45RA
+
Regulatory T Cell Isolation Kits from human blood or PBMCs.
1
The kit is also suitable for usage with rapamycin.
2
The Treg Expansion Kit is based on MACSiBead™ Particles pre‑loaded with CD3 and CD28 antibodies. Optimal expansion of Treg cells is accomplished by using pre-loaded MACSiBead Particles and Treg cells at a bead-to-cell ratio of 4:1 and recombinant interleukin 2 (rIL-2) with a concentration of 500 U/mL.

Downstream applications

Expansion of human Treg cells after isolation with the CD4
+
CD25
+
CD127
dim/–
or CD4
+
CD25
+
CD45RA
+
Regulatory T Cell Isolation Kits from human blood or PBMCs. Expanded Treg cells can be used for any downstream application such as cytokine analysis, gene expression, and suppression assays.
Product options: -

Product information

Size
Order no.
Price

Related products

4 products available