MSC Suppression Inspector, human

MSC Suppression Inspector, human

The MSC Suppression Inspector was developed for the functional characterization of human mesenchymal stem cells, also known as mesenchymal stromal cells (MSCs) by
in vitro
suppression assays.

Background information

MSCs are fibroblast-like plastic-adherent cells that can be isolated from a variety of tissues, such as bone marrow or adipose tissue. During the last few years the attention of scientists was redirected away from the multipotentiality of MSCs towards their possibility for immunomodulation. It was observed that bone marrow derived MSCs suppress T-cell proliferation
1,2
. This function of MSCs can be analyzed using the MSC Suppression Inspector which contains an optimal T cell stimulation reagent for an MSC suppression assay.

Detailed procedure

The MSC Suppression Inspector consists of Anti-Biotin MACSiBead Particles that are pre-loaded with biotinylated CD2, CD3, and CD28 antibodies. MSCs are co-cultured with CD4
+
CD25
or CD4
+
responder T cells (Tresp) at different ratios in the presence of a polyclonal stimulus, in this case the MSC Suppression Inspector. Tresp cells alone show a proliferative response. Co-culture of MSCs with Tresp cells results in reduced proliferation of Tresp cells. Cell proliferation is determined by
3
H-thymidine incorporation or detected by carboxyfluorescein succinimidyl ester (CFSE) staining.
Product options: -

Product information

Size
Order no.
Price

Related products

7 products available | view all