PepTivator Zika Envelope Protein E3

PepTivator Zika Envelope Protein E3

PepTivator
®
Zika Envelope Protein E3 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the sequence of the Zika envelope protein E (domain 3) from virus isolate Z1106033 polyprotein gene (GenBank Acc. no. KU312312.1).
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

The Zika envelope protein E (domain 3) from virus isolate Z1106033 polyprotein gene comprises the amino acids 588–796 of the full length polyprotein. The Zika virus belongs to the family of
flaviviruses
and is spread by
Aedes
mosquitos. In most cases no symptoms are observed after infection, but about 20% of Zika infected individuals react with symptoms like fever, red eyes, joint pain, and headache. During pregnancy the virus can spread to the fetus and cause microcephaly and other brain malformations. The virus is distributed in around 40 countries and the
Aedes
mosquitos can be found in all tropical and subtropical areas.

Downstream applications

PepTivator Zika Envelope Protein E3 – research grade has been specifically developed for efficient
in vitro
stimulation of Zika Envelope Protein E3–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications, including:
  • Detection and analysis of antigen-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable antigen–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of antigen–specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.
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