PepTivator WT1, human

PepTivator WT1, human

WT1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the WT1 protein (UniProt ID: P19544).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific T cells, as peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
and CD8
T cells in various applications.
Quantitative, phenotypical, or functional analysis of WT1–specific T cell immunity can provide important information on the natural course of immune responses.

Background information

Wilms tumor 1 (WT1), a zinc-finger transcription factor, is expressed during normal ontogenesis. Expression is absent or restricted to a very low level in adult healthy tissue. The WT1 protein is abundantly found in various types of hematological maligancies and solid tumors. It is a key molecule for tumor proliferation, inducing spontaneous humoral and cytotoxic T cell responses, and has become an attractive target for anticancer immunotherapy.

Downstream applications

in vitro
stimulation of WT1–specific T cells with PepTivator WT1 causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of WT1–specific T cells.
  • Detection and analysis of WT1–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable WT1–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines for research on immunotherapy.
  • Generation of WT1–specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
  • Pulsing of antigen-presenting cells for research on dendritic cell vaccination.
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