PepTivator Tyrosinase, human

PepTivator Tyrosinase, human

PepTivator
®
Tyrosinase is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of human Tyrosinase (UniProt ID: P14679).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative, phenotypical, or functional analysis of tyrosinase-specific T cell immunity can provide important information on the natural course of immune responses.

Background information

Besides gp100/Pmel17 and Melan-A/MART-1, tyrosinase belongs to the melanoma-associated antigens of the group of differentiation antigens. It is expressed on normal melanocytes, but is also present in melanoma. Accordingly, CD4
+
and CD8
+
T lymphocytes recognizing tyrosinase have been identified in melanoma patients. Therefore, tyrosinase represents a potential target for immunotherapy of melanoma
2
.

Downstream applications

The
in vitro
stimulation of tyrosinase-specific CD4
+
and CD8
+
T cells with PepTivator Tyrosinase causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of tyrosinase–specific T cells.
  • Detection and analysis of specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
  • Isolation of viable tyrosinase–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of tyrosinase–specific CD4+ and CD8+ effector/memory T cells for research on immunotherapy and vaccination.
  • Pulsing of antigen-presenting cells, for example, for research in dendritic cell vaccination.
Product options: 2

Product information

Size
Order no.
Price

PepTivator Tyrosinase - premium grade, human

  • For research use only
for
stimulation of
10
8
cells
130-094-445
-

PepTivator Tyrosinase - premium grade, human

  • For research use only
for
stimulation of
10
9
cells
130-094-446
-

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