PepTivator Survivin 1, human

PepTivator Survivin 1, human

PepTivator
®
Survivin 1 is a peptide pool that consists mainly of 15-mer peptides with 11-amino acid (aa) overlap, covering the complete sequence of the human survivin protein (UniProt ID: O15392).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative, phenotypical, or functional analysis of Survivin 1–specific T cell immunity can provide important information on the natural course of immune responses.

Background information

Survivin is a bifunctional protein that suppresses apoptosis and regulates mitosis. It is expressed during development in proliferating cells, but remains absent in differentiated tissue. In most human solid malignancies and in leukemia elevated levels of survivin are found contributing to the survival and proliferation of tumor cells. In cancer patients survivin-specific T cell reactivity has been observed. Thus, survivin is described as a candidate target antigen for immunotherapeutic interventions.
2

Downstream applications

The
in vitro
stimulation of Survivin 1–specific CD4
+
and CD8
+
T cells with PepTivator Survivin 1 causes the secretion of effector cytokines and the up-regulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of Survivin 1 –specific T cells.
  • Detection and analysis of Survivin 1–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
  • Isolation of viable Survivin 1–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of Survivin 1–specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
  • Pulsing of antigen-presenting cells.
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