PepTivator STEAP1, human

PepTivator STEAP1, human

PepTivator
®
STEAP1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human STEAP1 (Six-transmembrane epithelial antigen of prostate 1) protein. (UniProt ID: Q9UHE8) .
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

The 339 aa metalloreductase STEAP1 (Six-transmembrane epithelial antigen of prostate 1), is highly expressed not only in prostate tumors but also in bladder, colon, ovarian, and Ewing sarcoma cell lines.
1,2

Applications

PepTivator STAEP1 – research grade has been specifically developed for efficient
in vitro
stimulation of STEAP1–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications, including:
  • Detection and analysis of STEAP1–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable STEAP1–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of STEAP1–specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.
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