PepTivator HPV18 E6, human

PepTivator HPV18 E6, human

PepTivator
®
HPV18 E6 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human papillomavirus (HPV)18 E6 protein (UniProt ID: P06463).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific T cells, as peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
Quantitative, phenotypical, or functional analysis of HPV18 E6–specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.

Background information

HPVs are non-enveloped, double-stranded circular DNA viruses. Persistent infections with several HPV types are associated with the development of tumors. HPV types 16 and 18 together cause about 70 percent of cervical cancers. The viral E6 and E7 proteins of HPV16 and HPV18 are involved in the initiation and maintenance of oncogenic processes. HPV E6– and E7–specific T cell immune responses play an important role in successfully clearing HPV infection and controlling HPV-associated diseases.

Downstream applications

The
in vitro
stimulation of antigen-specific T cells with PepTivator HPV18 E6 causes the secretion of effector cytokines and the up-regulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of HPV18 E6–specific T cells.
  • Detection and analysis of HPV18E6–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable HPV18 E6–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of HPV18 E6–specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
  • Pulsing of antigen-presenting cells for research on dendritic cell vaccination.
Product options: 2

Product information

Size
Order no.
Price

PepTivator HPV18 E6 - premium grade, human

  • For research use only
for
stimulation of
10
8
cells
130-096-005
-

PepTivator HPV18 E6 - premium grade, human

  • For research use only
for
stimulation of
10
9
cells
130-096-006
-

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