PepTivator GAD65, human

PepTivator GAD65, human

PepTivator
®
GAD65 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of human glutamic acid decarboxylase (GAD) 65 (UniProt ID: Q05329).
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

GAD65 is expressed by pancreatic β cells and appears to be an important autoantigen involved in the onset and pathogenesis of type 1 diabetes.

Downstream applications

PepTivator GAD65 – research grade has been specifically developed for efficient
in vitro
stimulation of GAD65-specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications, including:
  • Detection and analysis of GAD65-specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable GAD65-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of GAD65-specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.
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